...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck... at small and large scale.  This new technique involves selection in the cytosol (instead of on the yeast...

...Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development and characterization of sindbis...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...219. PubMed PMID: 27280971. 10. Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency..., which is particularly well suited for strand invasion, a key step in the homologous recombination pathway...

...Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto...sample type. Sample Preparation This step can involve multiple smaller steps that all help to ensure ... Similar to Sample Preparation, this step can involve multiple smaller steps, but the aim is to preserve...

...Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen K , Lang E, Kaspar AA, Foust KD, Thomsen G, Fugere M, Kaspar ...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

...Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from ... plasmids encode a negative regulation system involving antisense RNAs or iterons that inhibits replication...

...transformed into AdEasier-1 cells , which are BJ5183 E. coli cells containing pAdEasy™. pAdEasy™ is a ∼33Kb...Useful for very large inserts AdEasier-1 cells BJ5183 E. coli cells containing the pAdEasy-1 packaging plasmid...renders the virus replication incompetent. E3 is involved in evading host immunity and is not essential ... replication incompetent E3 Non-essential gene involved in immune evasion, deleted to increase packaging...cell line Transfer Vocabulary Term Definition ITR Inverted terminal repeat; Contains elements that promote...

...Repeat) sequences were initially discovered in the E. coli genome in 1987, but their function as a safeguard...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others)...Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease... Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM...9. PMID: 26355004 Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, ...as a powerful defensive strategy against viral invaders, and this system is proving to be just as useful...CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy the phages upon re-...

... conferences and had real-time correspondence via e-mail throughout the entire year. In addition to their...base of former mentees, we encourage them to get involved and give back by becoming mentors to new students...

...Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins and mechanisms of miRNAs and siRNAs..., M., Rashno, M. M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions...Link opens in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., Taxman, D. J. (2010). Short hairpin... silencing mechanism. It is a useful tool to investigate the roles of specific genes or to knockdown genes...