...towards successful iEP reprogramming. MettI7a1 encodes an uncharacterized putative methyltransferase. ... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed...detected over a 10-week period. Their expression decreased over time but they were not silenced.    Find ...retroviral overexpression cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell...

...that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain antibody. To create their ... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...them. HRP RANbody staining results in a ≥10-fold increase in signal over staining with a primary/ labeled...

...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...to bind to a previously hidden epitope (right). Created withBioRender.com.   Biological Processes  ...

...cells to retain plasmids - if a cell does not inherit plasmid due to improper plasmid partitioning, it...unstabilized plasmids were retained in less than 10% of cells while plasmids containing the plasmid addiction...selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF contains...ensure that plasmids are passed down through generations (Figure 1). These so called “plasmid addiction ...only survive if they retain the plasmid. Since their discovery, plasmid addiction systems have been used...found that if a mini-F plasmid carried a specific 700 bp segment of DNA, the plasmid was propagated better...restriction-modification systems onto plasmids allowed cell populations to retain more of these plasmids as compared to those...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a barcode, and...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...head over to our CRISPR Plasmids and Resources page.  A new system uses light to deactivate photocleavable... accumulation of indels to measure time. These plasmids with different promoters driving expression of...

...Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection...The Seeger lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against...nanobodies can be synthetically expressed off of plasmids in bacteria, and they can target epitopes that...form of mRNA for academic research. The template plasmids for generating your own libraries are also available...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds...libraries. Each mRNA molecule in the library doesn’t encode a stop codon, which causes the ribosome to pause...efficiently subcloned into the phagemid pDX_init plasmid, using the fragment exchange (FX) cloning technique...

...for expression of a gene carried on the transfer plasmid or quantifying the number of viral copies that ...beneficial as they ensure that the correct transfer plasmid was used in the transfection; if preparing several... that comes from amplification of the original plasmid which can over-inflate results. A packing vector...number of target cells that are positive for vector encoded transgene expression following transduction with...antibiotic resistance genes carried on transfer plasmids. Target cells are transduced with serial dilutions... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

...Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...transposon system. This system has two parts: a plasmid expressing the Tn7 transposition genes (tnsA, tnsB... tnsB, tnsC, tnsD, and tnsE), and a plasmid that contains the Mobile CRISPRi cassette flanked by left ...copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during a tri-parental...Mobile-CRISPRi ICE element forms a conjugative plasmid-like DNA circle and is transferred to recipient...transposon containing the CRISPRi components and a plasmid with the Tn7 transposition genes are transferred...RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the ability to synthesize diaminopimelic...

...performed by transfecting cells with a plasmid, such as PX459, which encodes Cas9 and contains a site for inserting...chosen for both Cas9 and gRNA expression       Plasmid DNA may be incorporated into the genome     Off-target...targets with similar efficiency as compared to plasmid-based expression of Cas9/gRNA and can be used for... as CMV or EF1A promoters found in many CRISPR plasmids) are not expressed. Because this method does ...fluorophore tagged dCas9 may require the use of plasmid or viral-mediated delivery. You’ve decided to...and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab...experimental systems that may not be amenable to plasmid based methods. One of the most common techniques...

...the endogenous target, and thus no genetically encoded tag is required. These dyes can also be attached...® and HaloTag®. These consist of a genetically encoded enzyme variant tag that covalently reacts with ...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...More resources from Addgene Fluorescent protein plasmids and resourcesA practical approach to choosing ...fluorescent dyes, and fluorescent ligands. Figure created with biorender.com. Fluorescence refers to...fluorescent protein tags, such as higher specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...