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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 399 results for: MUT

Showing: 261 - 280 of 399 results

  4. p53 Pathway

    Type
    Collection
    ...frequently mutated gene (>50%) in human cancer; most of these mutations are missense mutations in the DNA-binding...hotspot residues. These mutations can be inherited or arise through exposure to mutagens like radiation or ...PubMed PMID: 24379683 . When mutants gain new powers: news from the mutant p53 field. Brosh R, Rotter V...19776742 . Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20... (e.g., HPV, the human papillomavirus). These mutations interfere with p53’s ability to activate transcription...Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function, expanding... expanding the mechanisms by which mutant p53 is known to function in cancer. p53 Pathway Plasmids Click...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

  6. Pooled Library Amplifications

    Type
    Blog Post
    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  9. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

  16. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...span the knock-in gene. For making mutations, one good way is to mutate the PAM 'NGG' sequence in the HR...a silent mutation. When attempting to use the CRISPR/Cas9 system to create specific mutations or insertions...possible. For introducing small mutations (<50bp) or a single-point mutation, the best HR template for transfection...the introduction of a mutation into the genome, how can cells with that mutation be selected/screened?...on each side flanking your desired insertion or mutation. Each arms is around 800bp. For transfection in...the HR template, you usually would need to make mutations of the HR template to avoid this donor plasmid...being degraded by Cas9 in cells. One good way is to mutate the PAM 'NGG' in the HR template, like change it...

  17. CRISPR Guide

    Type
    Collection
    ...developed by mutating specific amino acid residues to reduce off-target editing. Some mutations disrupt interactions...off-target mutations in DNA, RNA, or both, and are recommended in contexts where such mutations would be... and a gRNA will result in a diverse array of mutations (see: Validate genetic modification ). In most...amino acid deletions, insertions, or frameshift mutations leading to premature stop codons within the open...targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the strength...strength of the knockout phenotype for a given mutant cell must be validated experimentally. Browse Plasmids...(nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one...
Showing: 261 - 280 of 399 results