...standard curve RNase-free DNase 10X DNase buffer Nuclease-free water Microcentrifuge tubes 96-well optical...0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid...10 9 stock or previous dilution (μL) Volume of nuclease-free water (uL) Molecules per μL 10 90 2 x 10 ...Dilution Series Volume of sample (uL) Volume of nuclease free water (uL) Dilution factor Total dilution...

...microscopes, amplified staining methods, and oligonucleotide-based staining. Of course, if you’re interested...

...Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/...

...Baird says. From F-Actin to mitochondria, from the nucleus to cell membrane, this collection has fluorescently...

... a particular cellular compartment such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole...

...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...

...region with a high amount of guanine and cytosine nucleotides, we might need to send it for Sanger sequencing...

... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...

...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...

...Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity to the “dopamine...

... drive expression, is it necessary to add a G nucleotide to the start of my target sequence? A2: The human...

...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR...

... 5 minutes to allow for dissociation of the nucleoprotein complexes. The effectiveness of your RNA isolation...agarose gel, or bioanalyzer. For more information on nucleic acid quantification, see our protocol for DNA quantification...

...chemical fluorophores for your next experiment. Nucleic acid based machines Recombinase-based State Machines...

...with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors...

... find the ACE reporter plasmid! Martin et al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ...

...disease-causing point mutations. They allow single-nucleotide modifications to edit DNA without requiring a...

...Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...

...researchers used the HA frankenbody to successfully track nuclei in a developing zebrafish embryo over an 80 minute...

... nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics researcher on...