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We narrowed to 650 results for: CHI;

Showing: 391 - 420 of 650 results

  2. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again...starve while you are away a few days. Also, your children will enjoy day care and school if you tell them...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun ...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...

  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...plants." Plant Biology 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...

  7. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...hope that a similar reversal may be possible for children and adults with Rett Syndrome.  Since these discoveries...these approaches, such as delivery of the editing machinery to the brain, minimizing off-target editing, and...

  8. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review...Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Reddit Science is heavily moderated. I started watching the questions and comments go up early in the ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early in the...

  12. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    ...Grover M. Hutchins. "Henrietta Lacks, HeLa cells, and cell culture contamination." Archives of pathology...

  15. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...protein products, which can prevent the culture from reaching the desired density within a reasonable time-frame...cystosolic protein products, but this is not always achievable. Proteins which contain hydrophobic regions or...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...biotin, avidin. Avidin was aptly named for the chicken, “Avian”, egg it was purified from. Snell later...Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...

  18. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...or difficult to create this list. Start BEFORE searching for a faculty position. This is important to ensure...seem, you should consider it if it will help you achieve your research goals, even if that means saying ...

  21. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Although there are different ways to ultimately achieve success, we find it is usually faster and easier...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...

  22. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...need! How does it work? There are several ways to achieve multiplex IF. One of the most common approaches...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start...

  23. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...for mammalian expression and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral...Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed...

  24. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced...for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding sites within...

  25. Finding Your Perfect Job After University

    Type
    Blog Post
    ...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers... world. The research project was hard; I was researching the epidemiology of breast cancer and I loved...

  26. Finding Your Science Policy Path

    Type
    Blog Post
    ... target. There are a multitude of options for achieving success in a science policy career with many opportunities...organizations, working in student government, and teaching and mentoring others (see more in Figure 2). Starting...

  28. Typing CRISPR Systems

    Type
    Blog Post
    ... mammalian cells. Type IV Type IV is the middle child (like me!) of CRISPR types — weird and poorly understood...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...

  29. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...a slide, or whole organs. Because the tissue architecture is preserved, this technique allows you to not...

  30. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... inhibitor with a degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are ...plasmids from this paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for ...
Showing: 391 - 420 of 650 results