We narrowed to 658 results for: CHI;

...that you use the same solution for blanking your machine!  Finally, many NanoDrop systems now provide full-spectrum...are no substitute for sound techniques, routine machine maintenance, and critical interpretation of the...

...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...when interpreting findings, particularly when researching ancestry groups. And, of course, it’s imperative...

...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....experience.  Can combines cons of multiple medias. Switching from one media type to another is cognitively ...

...using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins...

...fluorophore can emit a limited number of photons before bleaching. The amount of information one can extract from...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...

...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...

... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again...starve while you are away a few days. Also, your children will enjoy day care and school if you tell them...

... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...plants." Plant Biology 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed...

...Strongylocentrotus purpuratus, the spiny purple sea urchin. It’s not a standard model organism, but it was...neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the smallest photosynthetic...

...hope that a similar reversal may be possible for children and adults with Rett Syndrome.  Since these discoveries...these approaches, such as delivery of the editing machinery to the brain, minimizing off-target editing, and...

...10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV...

...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...

...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...

...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun ...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...

...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review...Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your...

...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

...Reddit Science is heavily moderated. I started watching the questions and comments go up early in the ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early in the...

...Grover M. Hutchins. "Henrietta Lacks, HeLa cells, and cell culture contamination." Archives of pathology...

...the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to...Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus.   ...

...protein products, which can prevent the culture from reaching the desired density within a reasonable time-frame...cystosolic protein products, but this is not always achievable. Proteins which contain hydrophobic regions or...

...editing and longer genome search times. Using a machine learning approach, the lab proposed multiple sets...CRISPR-Cas9 PAM variants via scalable engineering and machine learning. Nature, 643(8071), 539–550. https://doi.org...

...biotin, avidin. Avidin was aptly named for the chicken, “Avian”, egg it was purified from. Snell later...Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...

...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires...within your lab? In every lab, there are certain hierarchies and restrictions to data access. If these are...

...or difficult to create this list. Start BEFORE searching for a faculty position. This is important to ensure...seem, you should consider it if it will help you achieve your research goals, even if that means saying ...

...opening the door for immunostaining analysis. To achieve even cell labeling, they generated farnesylated..., Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi:...

...Although there are different ways to ultimately achieve success, we find it is usually faster and easier...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...

...need! How does it work? There are several ways to achieve multiplex IF. One of the most common approaches...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start...

...importantly, it doesn’t display the problematic photoswitching upon blue illumination that plagues other red...mApple, which means it likely has the blue-light photoswitching issue). Although similar in brightness to jRGECO1a...

...for mammalian expression and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral...Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed...

...alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced...for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding sites within...