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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 395 results for: POR C

Showing: 391 - 395 of 395 results

  1. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...standard Before Starting Warm the hot plate to 100 °C. Thaw IgG standard and prestained protein ladder on...microcentrifuge. Heat the samples for 10 min at 100 °C in a heat block. Spin the sample briefly in the microcentrifuge...IgG standards in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: June 14...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select... each peak by selecting the center of the peak. Export results as a csv file. In the results table, select...

  2. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...longer period of time; b) using a wider gel comb; or c) loading less DNA in the well. How do you get better...around the band as possible. To do so, it is often important to take the excised band, lay it down on the UV...sides with the razor blade. This is especially important during the DNA purification step, as many kits... fragment. The weight of the gel is directly proportional to its liquid volume and this is used to determine...manufacturer's instructions. Note: It is usually important to determine the concentration of the DNA that...

  3. Plan Your Experiment

    Type
    Guide
    ...) may be more efficacious than gRNAs containing a C nucleotide at the same position in spite of being ...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...may alter protein function when they occur in important protein domains. For gene editing experiments ... addition to off-target activity , it is also important to consider factors that maximize cleavage of ...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...

  4. Water Bath Protocol

    Type
    Protocol
    ... refrigerators to achieve a temperature between 4°C and room temperature. This general protocol suits ...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

  5. Ligation Independent Cloning

    Type
    Protocol
    ...software to ensure a melting temperature between 50-60°C for your PCR primers. Step 2: Linearize Vector In ...provided by your polymerase manufacturer. It is very important to remove all free nucleotides from your PCR product...
Showing: 391 - 395 of 395 results