We narrowed to 621 results for: Emb

1. CRISPRainbow and Genome Visualization

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   ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

2. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

3. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

4. The Future of Research Symposium Boston 2015

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   ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

5. New Tool for Lineage Tracing: The ClonTracer Library

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   ...library allows a researcher to label individual members of a starting population of cells with specifc,...

6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

7. Viral Vectors 101: Virus Safety

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   ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

8. Antibodies 101: Choosing the Right Isotype

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   ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

9. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

11. Engaging with science and society at pgEd

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    ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

12. Overcoming the Challenges of Lentiviral Production

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    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

13. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

14. Adenoviral Vector Production and Troubleshooting

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    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

16. Corralling and Cornering the Goldilocks Antibody

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    ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

18. CRISPR in the Clinic

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    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

19. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

20. 6 Tips for Grant Writing

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    ...about what reviewers in your area are looking for. Remember though, you need to leave them time to read it...

21. Tips for Technical Support Calls

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    ... money on a potentially faulty product. Please remember that the person at the other end of that email...

22. Common Injection Routes in Mice

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    ...When considering what method you’d like to use, remember that the final decision will likely come down ...

23. Pooled Library Amplifications

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    ... protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol! Each...

24. Antibodies 101: Flow Compensation

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    ...But we hope this guide can help you get started. Remember, you can always change your compensation values...

25. Of Myc and Men

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    ...mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984...

26. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae...

27. 3D Printing Meets CRISPR Cas9

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    ...this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students...

28. Transferable Skills: Negotiation

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    ...colleagues and supervisors and lead to you being remembered as a person who tries to find solutions that ...

29. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to prey upon...

30. Truncated gRNAs for Regulating Gene Expression

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    ... environments.     Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory...

31. Addgene's Cancer Collection Pages Connect the Research Community

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    ... We invite feedback and contributions from all members of the research community as we continue to to ...

32. Experimenting with New Careers while in Grad School

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    ...positive. I went back into lab the next day and remembered why I still love science. Keep building on your...

33. Anatomy of a Plasmid Page at Addgene

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    ...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify...

34. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified...

35. Antibodies 101: Producing Recombinant Antibodies

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    ...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (...

36. CRISPR 101: Making a Knock-In Cell Line

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    ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

37. SciComm: Taking Science to Elementary and Middle Schools

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    ... Science Night, a chance for parents and other members of the community to share their expertise with ...

38. AAVs in Retinal Gene Therapy

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    ...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

39. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...commitments, respond to any issues raised by team members, and plan for the future of the team. All while...

40. Summer SciComm Series: Modes of Communication

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    ... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind...