We narrowed to 642 results for: Emb

...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

...library allows a researcher to label individual members of a starting population of cells with specifc,...

...Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling...

...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

...about what reviewers in your area are looking for. Remember though, you need to leave them time to read it...

... money on a potentially faulty product. Please remember that the person at the other end of that email...

...Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the collection and order individual...

...this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students...

... environments.     Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory...

...colleagues and supervisors and lead to you being remembered as a person who tries to find solutions that ...

...principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae...

...allows VAMP2-At to remain on the presynaptic cell membrane while AF, fused to a payload, is released into...

...When considering what method you’d like to use, remember that the final decision will likely come down ...

... protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol! Each...

...But we hope this guide can help you get started. Remember, you can always change your compensation values...

...mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984...

...camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to prey upon...

... We invite feedback and contributions from all members of the research community as we continue to to ...