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We narrowed to 849 results for: HA

Showing: 521 - 560 of 849 results

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...ends interfere with that step. Accordingly, NHEJ has a vast toolbox of processing factors, including polymerases...

  4. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...base editing to pooled library screening. It also has information for planning your CRISPR experiment. ...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...including creating Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method...left homology arm guanine (i.e. GG); this design has been shown to increase T7 promoter activity. To avoid...

  6. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...served by a one-sample test as the standard value has no variation that needs to be accounted for. ANOVA...multiple range test, and several others. Each test has different likelihoods of returning false positives...to a single group. Dunnett’s is more powerful and has a lower error rate than the multiple comparison tests...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...prime editing By Emily P. Bentley The David Liu Lab has made two recent deposits focused on optimizing prime...collaboration with the Vallabh/Minikel Lab, the Weissman Lab has developed a new epigenetic editor, Coupled Histone...subtypes, and a familiar challenge for anyone who has done histology on brain tissue is identifying the...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...well if you’ve been following the Addgene blog, it has been repurposed for genome editing in eukaryotes,...editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (Zhang et al., 2016...than that, be nice to your microinjectionist, who has the strategically important job of delivering the...

  9. Deep Dive: qPCR

    Type
    Blog Post
    ... a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while...for all amplicons; if your amplicon is AT-rich or has a secondary structure, you may see a double peak ...being a common choice - the frequently used GAPDH has noted issues as a reference gene (Higgins, 2000; ...assumptions that match your data set, that is.) Yuan, 2006 has a good overview of different statistical analysis...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...Retroviridae family. Infection with lentiviruses has a long incubation period that eventually leads to... genes, mutations, or treatments into cells, and has been widely used in the research community for decades...VSV-G is by far the most common envelope gene, as it has a wide range of infectivity across many cell types...their stable expression and wide tropism. Addgene has an extensive collection of lentiviral CRISPR libraries...

  11. Molecular Biology Reference

    Type
    Guide
    ...isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant DNA technology...isolates, the K-12 strain and the B strain. K-12 has led to the common lab strains MG1655 and its derivatives...polymerase. Thus to replicate a piece of DNA in vitro one has to know some of its sequence to design a effective...Epitope Tags Tag Amino Acid Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST...

  12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...women. Bicuspid aortic valve (an aortic valve that has two leaflets, instead of three) and acute aortic ...intracellular signaling,” says Hébert. Previous research has shown that GNB1 mutations lead to increased activity...

  13. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...in the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...easy favor for people in your network. If someone has connected with me, I consider them fair game for ...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...-homologue recombination was released. This blog has not been updated in response to this paper. Any hint... hint of CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published...

  15. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...The characterization of RNA-protein interactions has lagged behind, likely due to limitations of current...interactions. Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...Cecchetelli The auxin inducible degron (AID) system has been extensively used in C. elegans since 2015 to...light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA...

  18. To Each HIS Own

    Type
    Blog Post
    ...can be pretty complicated given that a single cell has tens of thousands of proteins functioning in a variety... would not be able to do. We hope that this blog has answered all of your questions about polyhistidine...

  19. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... genes, mutations, or treatments into cells, and has been widely used in the research community for decades...VSV-G is by far the most common envelope gene, as it has a wide range of infectivity across many cell types...promoter; the transcript begins at the beginning of R, has a 5' cap structure, and proceeds through U5 and the...

  20. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... CRISPR 101: Una herramienta de edición  Addgene has a large international audience, including working...

  21. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Further, Sanger sequencing has a lower detection limit of 50-20% (although this has been improved in some ...Joung 2016 with permission. Even though progress has been made with off-target prediction algorithms, ...

  22. Hot Plasmids - October 2020

    Type
    Blog Post
    ...generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory...engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene...

  30. A Look at Addgene's QC Process

    Type
    Blog Post
    ...including the propagation of plasmids in bacteria — has many points at which small errors can be introduced...Catching and addressing QC issues Addgene’s QC team has developed a process for identifying, categorizing...

  31. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...categorization is complex Plasmid classification has shifted over the years, and new classifications are...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many...

  32. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab. Additionally...important — but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available...

  33. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...syndrome. In response, the World Health Organization has declared a global health emergency and called for...molecular and electronic companion technologies that has transformed the assay’s capabilities. Specifically...

  36. Pouring LB Agar Plates

    Type
    Protocol
    ...darken during the autoclave process if your sample has spent at least 10 min at 121 ℃. Use lab tape to label...

  37. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...
Showing: 521 - 560 of 849 results