We narrowed to 877 results for: HA

...slower, more traditional, QC process. While this has worked well for the early sets of SARS-CoV-2 materials...certain processes in both core operations. As it has been in much of the world, we have found ourselves...

...time Since the publication of our paper, our group has been inundated with requests to share our plasmids...). Also, we’ve got to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, ...

...science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often than not...these tools and to tell us how useful the service has been for their experiments. This helped reinforce...

...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification.... Using the same example plasmid as above (which has the XbaI site blocked by methylation), the figure...

...disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind...

...reagents. F1000Research, among many other journals, has joined a pilot project from the Neuroscience Information... up their own minds on whether the data analysis has been of good quality and whether they agree with ...

...and the master plasmid in one step. The kit also has other unique aspects that make it especially well-suited...for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be more efficient...

...dye, but has been modified with the addition of two iodine residues. Compared to MG, MG-2I has increased...

...analytical ultracentrifugation yields an affinity and has been used to demonstrate that yellow fluorescent ...structure. Based on these data, we conclude that mKOk has dimerization tendency and is not suitable for protein...

...well or cannot be detected in a microscopy setup has zero practical brightness and is of no use. Therefore... practical brightness can be made. This strategy has been used in yeast by Lee et al (2013) and in nematodes...

...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...light of a different color instead. This approach has even been used in biosensors where BRET reports on...

...gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging...components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning X gene, an ...

... genes of interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate...rounds of replication are initiated before the cell has divided. This means that genes located near the origin...

...Singer’s lab at Albert Einstein College of Medicine has recently published techniques to follow translation...Rojas! Eugenia Rojas, PhD is a neuroscientist who has always been deeply interested in neurobiology.  She...

...hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine...been approved by the FDA, and this mode of delivery has a lot of clinical promise moving forward. What viruses...

...immune response machinery of the host. This pathway has been clinically exploited for antiviral purposes ...deplete expression of proteins of interest. RNAi has even made it into agriculture as species-specific...

...For example, the Levin lab from Tufts University has used Arch (green-light activated proton pump) and...Allen Discovery Center. The future of this field has a high potential for the advancement of research ...

... Archon1 is  bright, exhibits good localization, has high signal:noise ratio, high sensitivity, responds...levels are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning system to generate a series...

... amounts or if the conjugated signaling molecule has a weak signal. For flow cytometry, secondary antibodies...introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal strength...

... - typically within 2-4 months. After the animal has the desired level of antibodies in its bloodstream...their batch to batch variability. Once an animal has undergone a terminal bleed to acquire all of its ...

...which is now easily available through Addgene and has already been requested dozens of times in 2 weeks...Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain, making...

...editor in 2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities...contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...

...easily assemble multiple DNA fragments. This system has been used to construct plasmids for use in model ...Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been recognized for its biotechnological...

...on holistic, community-centric science, our team has continuously engaged with a variety of stakeholders...Undergrad category at the iGEM Competition 2019. He has an active interest in scientific writing and science...

... human suffering and death. Doing research right has the potential to save lives and money. A 2014 analysis...physiologic or pathologic processes. Much progress has been made in this area in recent years, and you can...

...Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor...allosteric inhibitors at Novartis. The ClonTracer library has been distributed all over the world and was recently...

...functional elements in the human genome. The pair has focused considerable attention on transcription factors...the tagged transcription factor. The FLAG antibody has been well characterized and the system allows you...

...HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...ends interfere with that step. Accordingly, NHEJ has a vast toolbox of processing factors, including polymerases...

...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...can be manipulated at one time. Weissman’s group has made standard protocols available and they are working...

...base editing to pooled library screening. It also has information for planning your CRISPR experiment. ...

...prime editing By Emily P. Bentley The David Liu Lab has made two recent deposits focused on optimizing prime...collaboration with the Vallabh/Minikel Lab, the Weissman Lab has developed a new epigenetic editor, Coupled Histone...subtypes, and a familiar challenge for anyone who has done histology on brain tissue is identifying the...

...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...including creating Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method...left homology arm guanine (i.e. GG); this design has been shown to increase T7 promoter activity. To avoid...

...served by a one-sample test as the standard value has no variation that needs to be accounted for. ANOVA...multiple range test, and several others. Each test has different likelihoods of returning false positives...to a single group. Dunnett’s is more powerful and has a lower error rate than the multiple comparison tests...

...well if you’ve been following the Addgene blog, it has been repurposed for genome editing in eukaryotes,...editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (Zhang et al., 2016...than that, be nice to your microinjectionist, who has the strategically important job of delivering the...

... a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while...for all amplicons; if your amplicon is AT-rich or has a secondary structure, you may see a double peak ...being a common choice - the frequently used GAPDH has noted issues as a reference gene (Higgins, 2000; ...assumptions that match your data set, that is.) Yuan, 2006 has a good overview of different statistical analysis...

...-homologue recombination was released. This blog has not been updated in response to this paper. Any hint... hint of CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published...

...The characterization of RNA-protein interactions has lagged behind, likely due to limitations of current...interactions. Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding...

...can be pretty complicated given that a single cell has tens of thousands of proteins functioning in a variety... would not be able to do. We hope that this blog has answered all of your questions about polyhistidine...

...in the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...easy favor for people in your network. If someone has connected with me, I consider them fair game for ...

...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...