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We narrowed to 954 results for: App;

Showing: 651 - 700 of 954 results

  1. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this...

  3. Weighing Reagents Protocol

    Type
    Protocol
    ...the basics of how to weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to ...contain the reagent while on the balance, and an appropriately sized container to put the reagent in. For some...container. Dispose of the excess material into an appropriate waste container if the material is biohazardous...biohazard solid waste container. Conclusion Appropriately weighing reagents is important to ensure the...

  6. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...

  8. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

  11. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...

  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody...

  14. How Addgene Creates Content

    Type
    Blog Post
    ...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image...

  20. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of...

  23. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...

  24. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...working in the lab. Do not eat, drink, chew gum, or apply makeup in the laboratory. Before you start your ...and uncluttered. Disinfect your workspace with appropriate materials. For example, you may use 70% ethanol...your lab supervisor what is available and the appropriate contact times for each agent. Make sure you have...autoclaved. Conclusion Although simple, following appropriate BSL-1 and BSL-2 protocol goes a long way. It ...

  27. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

  28. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this...

  29. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make...

  30. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

  31. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing...

  32. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

  33. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...

  34. To Each HIS Own

    Type
    Blog Post
    ...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

  35. All in a Twist: dsRNA

    Type
    Blog Post
    ...RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail, diseases and...Fragile X syndrome, etc.). Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting...

  36. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  39. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events...

  40. Hot Plasmids - October 2020

    Type
    Blog Post
    ... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

  42. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer...

  43. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ...

  47. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...

  48. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology...

  49. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

  50. Tips for Technical Support Calls

    Type
    Blog Post
    ..., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene...
Showing: 651 - 700 of 954 results