Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 964 results for: app

Showing: 651 - 700 of 964 results

  1. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill...

  2. The MTA at Addgene

    Type
    Blog Post
    ...years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways...

  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day...

  5. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies...

  8. How to Lead a Great Meeting

    Type
    Blog Post
    ...assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people...

  9. Components of CRISPR/Cas9

    Type
    Blog Post
    ...gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications.   References Refactoring and optimization ...

  11. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...Chromosome Organization Keystone Symposium. As it happens, there are a lot of scientists who not only remember...

  17. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...

  18. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  19. How Addgene Creates Content

    Type
    Blog Post
    ...antibody and content team, we identified what applications should be included and what basic information...we usually make figures in BioRender or use appropriately licensed and cited figures from papers or image...

  20. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  29. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome. Non-homologous...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of...

  32. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...

  33. CRISPR Library Amplification

    Type
    Protocol
    ...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as... of a library of 100,000 plasmids. Colonies may appear small and require extra incubation time in order... as the quantification method. If all Maxipreps appear to contain sufficient DNA for use, pool samples...purifications. Consult the manufacturers handbooks for appropriate volumes and numbers of tandem purifications. ...

  34. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...

  35. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

  37. To Each HIS Own

    Type
    Blog Post
    ...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

  38. All in a Twist: dsRNA

    Type
    Blog Post
    ...RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail, diseases and...Fragile X syndrome, etc.). Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting...

  39. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  41. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

  42. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this...

  43. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

  44. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing...

  45. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make...

  48. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events...

  49. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology...

  50. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...
Showing: 651 - 700 of 964 results