We narrowed to 829 results for: cell

...occurs when bacterial cells regulate gene expression in response to changes in cell density. At the mixer...

... sure that the world will go on without my whole-cell extract from 2009. Join a newsletter to stay up-...

... that your reference genes are suitable for your cell lines and/or tissue types.  Please do note my oh-so-subtle...reference genes to save on plate space. For many cell lines and tissue types, appropriate reference genes...at determining the best options for a particular cell or tissue type (such as here, here, or here).  If...your data  Once you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want ...

...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org...

...is immediately ready to transform into competent cells. The master mix of enzymes can be purchased from...mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically ...

...introduction of nucleic acids into bacteria or eukaryotic cells is a common technique in molecular biology and scientific...

... mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient.  ...proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, ...while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the proprietary...expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose) or increased...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...Membranes The two membrane options are PVDF and nitrocellulose. PVDF is a sturdy membrane that can be stripped...membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You can visualize proteins...

...R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below. With these...transfecting the plasmids into mammalian tissue culture cells (e.g., HEK293, COS, CHO), from which the R-mAbs ...level expression we recommend using the HEK-2936E cell line engineered by Durocher et al., 2002 (Link opens...used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species...Pink1 Human M. musculus (mouse) IgG2b 227033 SynCAM1 cell adhesion molecule (Mus musculus) recombinant mouse...used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species...to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species...

...Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016/j.molcel.2015.04.025...

...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

...limit of detection for arg1 expressing ECN is 109 cells/ml, which is within the range of  the ~1011 commensal...

...SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks to quickly bring...

...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

...tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific...

...anti-tumour therapy using antibodies targeting dendritic cells. These genetic tools were pivotal in carrying out...

...recently shared our protocol for making competent cells to save time and money. My other stingy science ...

...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...

...antigens in living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in ...sdAbs over conventional antibodies include:  Excellent tissue penetration, thanks to their small size...

...al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before...

... repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX Knowledge Base: A ...

...experiments to test nickase preferences using HEK293 cells. They started by comparing D10A and H840A total ...

... expression after AAV injection, what region and cell class ended up expressing your virus? If you were...

... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...

...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

...a FRET donor to mCitrine and mOrange or for live cell imaging of fusions and FRET reporters. mTFP1 is ...

...fellow at Vanderbilt University. She studies beta-cell function and failure in Type 2 Diabetes. Birmingham...BC (Canada). She works in a lab that uses yeast cells to study how DNA damage promotes cancer. She is ...

...and chemically excited a class of enteroendocrine cells within the intestines known as neuropods. They observed...

...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...

... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...

..., and also a Professor of Molecular Genetics and Cell Biology at the University of Chicago. He was an ...

... small amount of spurious DNA from the packaging cell line, helper and packaging plasmids. We decided ...

...efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321...

...recommend validating the serotype in your target cells before conducting the experiment. Likewise, a lot...

...example, several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs) which...

...sciences, viewed through the lens of development, cell, and molecular biology. Beyond that, she is also...

... Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol for this...

...systems may prove effective in other species or in cell culture. In addition to CRISPR/Cas9 applications...

...devise new strategies to reproduce within a host cell. A few virus particles have learned to circumvent...

...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...

...day was Julie from Novartis who was working with cell culture and who was extremely helpful since she ...

...modification may not be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression ...

...spend the afternoon lovingly tending to the stem cells I was using in an experiment, then spend the evening...

... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry...

...manuscripts submitted for publication. Holds a PhD in Cell Biology and a Masters degree in Infection and Immunity...

... of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Brandon Harvey 137164 pAAV-nEF-Con/Fon/...

...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...

...industrial or research purposes requires stable host cells (the simplest example would be a transformed mesophilic...

...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...

...CRISPR-Cas12b for mammalian genome engineering." Cell discovery 4.1 (2018): 63. PubMed PMID: 30510770....