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  1. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers to... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...

  2. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...stock. All of our proceeds go into running the repository and serving scientists better. That includes ...reagents Addgene makes it easy for scientists to deposit and request plasmids (via web interface, processes...

  4. An Addgene Summer Internship

    Type
    Blog Post
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...

  6. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus...of the most popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting...

  9. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...Resources Learn More about Viral Vectors Browse the Repository  ...

  11. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx...

  12. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with all ...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...

  13. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not...

  14. Hot Plasmids - January 2023

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...

  15. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

  16. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...ability to infect cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive...Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...
Showing: 261 - 280 of 648 results