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Showing: 261 - 280 of 655 results
  1. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...scientists on the team ensure the deposits we received are what the depositing labs intended. With the use ...
  2. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ... yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ...
  3. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers to... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...
  4. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...stock. All of our proceeds go into running the repository and serving scientists better. That includes ...reagents Addgene makes it easy for scientists to deposit and request plasmids (via web interface, processes...
  5. An Addgene Summer Internship

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    Blog Post
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...
  6. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...
  7. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus...of the most popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting...
  8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...
  9. A Tour of Addgene's Most Popular Pooled Libraries

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    Blog Post
    ...Last month, the David Root and John Doench labs deposited new and improved libraries called Julianna, for...knockout screens. The Jonathan Weissman lab has deposited genome-wide CRISPR activation (CRISPRa) libraries...
  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...Resources Learn More about Viral Vectors Browse the Repository  ...
  11. Hot Plasmids: FRET-Based Biosensors

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    Blog Post
    ...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx...
  12. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with all ...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...
  13. Hot Plasmids - January 2023

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...
  14. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not...
  15. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ... fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...
  16. Bacterial Expression Systems

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    Collection
    ...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...
Showing: 261 - 280 of 655 results