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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 637 results for: ESP

Showing: 261 - 280 of 637 results

  1. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...

  2. Components of CRISPR/Cas9

    Type
    Blog Post
    ...is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...rate than that of bacteria (~24 h vs 20 min, respectively). Therefore, it is not always mission critical...plasmids containing the viral EBV or SV40 ORIs, respectively. The presence of these viral components greatly...

  6. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... a patient mutation to assess how patients may respond to drug treatment. Another example is using a mouse...gene expression temporarily. RNAi libraries are especially useful if you are interested in studying genes...

  7. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...made a huge difference in my ability to focus, especially on days when I was tired, stressed, or rushing...Try a variety of things, including (and maybe especially) suggestions you are initially resistant to. ...

  8. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...and others have used Twitter professionally. I especially love Dan’s advice to tap into the online conversations... tips for getting the most out of the site. I especially like the pointers on how to use LinkedIn to research...

  11. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... their decision. If you do object, do so in a respectful and professional manner. Arguing will make you...is a Developmental Editor for F1000Research, responsible for assessing the scientific content of manuscripts...

  16. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...request to any of these people, including and especially the hiring manager. This is considered intrusive...own good ideas, events and favorite resources. Respect the network and it will reward you. Resources ...

  17. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...a slash, that indicate the ITR and serotype, respectively. For example, AAV2/8 has a type 2 ITR and a ...serotype selection. Promoters and serotype control, respectively, cell-specific gene expression and viral infection...

  18. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...boxB Magenta boxB-MS2 White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently...fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs of identical hairpin...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    ...www.addgene.org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online...2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene pivoted ...

  20. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...was released. This blog has not been updated in response to this paper. Any hint of CRISPR editing in human...still be helpful in increasing viable embryos, especially in the case of older mothers, for whom egg retrieval...
Showing: 261 - 280 of 637 results