We narrowed to 475 results for: FLU

1. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   Blog Post

   ...will not produce the fluorescent protein while areas that are exposed will fluoresce. With these images,...

2. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   Blog Post

   ...on a molecular level. The brain is constantly influenced by signals it receives from the entire body, ... light emitting diodes, thermal sensors, and microfluidic channels. These features enable the fibers to...

3. Identifying Sequence Elements with SnapGene's Feature Database

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   Blog Post

   ... a feature inaccurately. The best example is fluorescent proteins, which often come in closely related...misidentification, we augment the database with new fluorescent protein variants. The future of DNA feature ...

4. New and Upcoming Viral Vectors - June 2019

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   Blog Post

   ...state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...

5. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ...amplify each TF to determine how its presence influences cell fates. The atlas of directed differentiation...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...

6. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   Blog Post

   ...plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya...

7. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ... relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...

8. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   Blog Post

   ...this by changing the voltage for the particular fluorophore prior to recording data. Increasing the voltage...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   Blog Post

   ...including FokI endonuclease domain as well as fluorescent proteins used to sort the cells after the plasmids...neurons, for instance – to see how those mutations influence the disease process. In the Cell Stem Cell paper...

10. Optogenetics Plasmid Collection

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    ...Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial Opsin Plasmids Optical...neuronal activity, scientists generally attach a fluorescent protein to the opsin in order to track its expression...

11. SARS-CoV-2 Pseudotyped Virus

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    ... below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral ...

12. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post

    ...in HeLa and MEF cells. For further reading on fluorescent microscopy techniques, the first author of this...El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4...

13. Antibodies 101: Affinity Reagents

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    Blog Post

    ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

14. Synthetic Biology - Overview

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    ...Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...

15. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ...that the method of Cas9 and gRNA delivery can influence the level of off-target effects, with electroporation...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

16. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV...promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only...

17. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...for monitoring homemade microfluidic chips using the fantastic optics and fluidics platform it contains....

18. Antibody Production

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    Collection

    ...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...

19. 10 Basic tips for mammalian cell culture

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    Blog Post

    ... more than 80-85% confluent because cells like their space. If they reach confluence, they stop growing...

20. Plasmids 101: Degron Tags

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    Blog Post

    ...for visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc...there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron (AID...