We narrowed to 342 results for: GAI

1. Google Forums Round Up: First Impressions of NgAgo

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   ... and development before it can truly be pitted against CRISPR head-to-head.  Exciting features of NgAgo...

2. Genome-wide Screening Using CRISPR

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   ...experiment can shed light on the mechanism by which cells gain resistance to drugs and can identify future therapeutic...

3. Tips for Using BLAST to Verify Plasmids

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   ...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...other words, these parts can be used over and over again in combination with novel genes to get very precise...

5. Getting to Know Expansion Microscopy

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   ...arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses, centrosomes...

6. A Guide to Starting Your Own Journal Club

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   ...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

7. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   ...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...

8. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...

9. Tips for arabidopsis transformation

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   ... let them recover for a week, and then dip them again.  This won’t hurt them, and it will increase the...

10. Simplify Cloning with in vivo Assembly

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    ...et al., 1993). But why did this technique fail to gain traction? It is likely that both vector amplification...

11. SciComm: Taking Science to Elementary and Middle Schools

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    ...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...

12. Negative Can Be Positive: Open AAV Data with Addgene

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    ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...

13. AAVs in Retinal Gene Therapy

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    ...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...

14. An Introduction to Adenovirus

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    ... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...

15. Viral Vectors 101: The Retroviral Lifecycle

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    ...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...

16. Great Results Start with Great Standard Curves

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    ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...

17. Antibodies 101: Producing Recombinant Antibodies

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    ... density of the antibody bands can be measured against the protein standard and the concentration may ...

18. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...have mastered the applications they use, you’ve gained experience working with similar protocols, and ...

19. Lentiviral Vector Uses and Overview

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    ...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...

20. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...separate plasmids), there are a few reasons arguing against this approach. First, this would prevent the study...