We narrowed to 344 results for: GAI

1. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...

2. Cloning Mammalian Cells with the Agarose Method

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   ...We test the (capped) 1:1 agarose/DMEM solution against a part of the body very sensitive to heat- the ...

3. Genome-wide Screening Using CRISPR

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   ...experiment can shed light on the mechanism by which cells gain resistance to drugs and can identify future therapeutic...

4. Tips for Using BLAST to Verify Plasmids

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   ...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...

5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...other words, these parts can be used over and over again in combination with novel genes to get very precise...

6. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...

7. A Guide to Starting Your Own Journal Club

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   ...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

8. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   ...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...

9. Negative Can Be Positive: Open AAV Data with Addgene

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   ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...

10. SciComm: Taking Science to Elementary and Middle Schools

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    ...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...

11. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...have mastered the applications they use, you’ve gained experience working with similar protocols, and ...

12. An Introduction to Adenovirus

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    ... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...

13. Viral Vectors 101: The Retroviral Lifecycle

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    ...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...

14. Getting to Know Expansion Microscopy

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    ...arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses, centrosomes...

15. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ... off starting from the beginning. Before trying again, revisit your purification protocol. Are any of ...

16. Great Results Start with Great Standard Curves

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    ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...

17. Antibodies 101: Producing Recombinant Antibodies

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    ... density of the antibody bands can be measured against the protein standard and the concentration may ...

18. Tips for arabidopsis transformation

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    ... let them recover for a week, and then dip them again.  This won’t hurt them, and it will increase the...

19. Simplify Cloning with in vivo Assembly

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    ...et al., 1993). But why did this technique fail to gain traction? It is likely that both vector amplification...

20. AAVs in Retinal Gene Therapy

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    ...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...