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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 261 - 280 of 856 results

  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...   Fig. 1: SpCas9 has sparse PAM coverage in the human genome. SpRY Cas9 has PAM sites covering the... a PAM with no sequence constraints, or at least has looser or different requirements. Luckily, there ...alternative PAM requirements (Zhao et. al, 2023). xCas9 has been shown to be less effective at NGH PAMs, though...

  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ..., full spectrum flow cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing...for PE. In order to correct for this, compensation has to be applied to “subtract” this spillover and correctly...they can more easily be detected. Cytek’s website has a convenient tool, called the similarity index, which...beads or cells. Overall, spectral flow cytometry has the ability to generate incredible amounts of data...

  3. Lab Automation at Addgene

    Type
    Blog Post
    ...Automating bacterial transformation One protocol that has been automated is high throughput transformation....benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different types ... how long to keep it there. In addition, the FxP has two robotic, mobile pods - one pod can pick up 96...cap sealer. This is a simple table top device that has a roller to go over caps and push them into micronic...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... development and function. To date, gene therapy has been used to treat 10 infants with this disease. ... nucleic acid cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies...AAV is not associated with any pathogenicity and has a low immunogenic profile. AAV was first discovered...Harshana S De Silva Feelixge is a researcher whose work has focused on gene therapy for viral infections. She...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ... transcriptional read-through. Chris Voigt’s lab has characterized a set of prokaryotic terminators and...for eukaryotic termination are understood and it has been noted that each eukaryotic RNA polymerase terminates...combination with certain viral packaging systems has been associated with reduced viral titer, but improved...Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to Reduce Viral Titer in a Promoter-Dependent...

  6. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...to see if anyone has published a protocol that would work for your system. Addgene has depositor submitted...Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing purposes because...Mammalian CRISPR expression systems Each model system has its own best practices for efficient delivery of ...

  7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...understands (synthetic biology), building a product no one has ever seen before (flowers that change color throughout...but the experience is invaluable. This opportunity has given us the chance to develop the technology we ...the business training to make the most of it. It has also allowed us to really analyze what we want out...Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors and experiencing...

  8. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...measuring DA and other neurotransmitters in the brain has been in vivo microdialysis of extracellular fluid... dLight1 dopamine sensor In 2018, Lin Tian’s lab has developed dLight1, a molecular tool to detect and...here! The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy...

  9. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...AAV5  pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) 121538  AAV5  pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 119741  AAV5 ...iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G...

  10. Hot Plasmids - October 2022

    Type
    Blog Post
    ...channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm and two-photon excitation around 1,040 nm, has larger photocurrents...anti-c-Myc [9E10] (Even, et al., 1985), the Myc tag has become one of the most commonly used protein tags...

  11. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...require heavy optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating...showed that the receptor-based fluorescent biosensor has a high sensitivity (50 nM-500nM range) and a high...recent months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use...

  12. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended... antibody libraries to generate new sdAbs. There has also been some interesting work done developing transgenic...al., 2021.   The single-domain antibody field has come a long way since the serendipitous discovery...

  14. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...KSgenome and seeing the intricacies of DNA repair has emphasized how essential these studies are to improving...alternative non-homologous end joining (Alt-NHEJ), has been harnessed for CRISPR knock-in applications. ...29266098 Creating knock-in mouse models with CRISPR has been challenging, with protocols using dsDNA templates...

  15. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...for mapping where a protein binds genome-wide, and has been widely applied in many model organisms and cell...proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse IgG, it is helpful to incubate...prior to the addition of Protein A MNase. Once MNase has been localized to target sites, the nuclease is briefly...

  16. The Strength of Story Telling

    Type
    Blog Post
    ...Show that as the story has progressed, you or your lab or science or your CV has grown as well. Find your...Task, Action, Result) interview technique, which has been addressed in much better pieces than I’m able...

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...inhibitory GABA-ergic interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements...fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed a synthetic red fluorescent protein... imaging and in protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. ...efficiency Blue flame depositor Yannick Doyon’s lab has developed a new method to increase CRISPR editing...increases the probability that the locus of interest has also been edited! Thus, ouabain selection enriches...coselection are available at Addgene, and the Doyon lab has also provided a detailed protocol at Protocol Exchange...Essential gene Knockdown library. Although B. subtilis has a fully sequenced genome, many applications in biotech...

  18. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders...broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin ...

  19. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme...one found on the left side of the image below. It has a promoter (blue arrow) followed by the restriction...finished plasmid   Once it looks like your ligation has worked, you will need to pick individual bacterial...purpose. Ideally, once you know that your plasmid has an appropriately sized insert, you should send it...plasmid for more details. Once your complete plasmid has been verified, you’re ready to get experimenting!...

  20. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...the growing spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization...sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists to tag and light up endogenous..., and T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The...characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for tagging endogenous...
Showing: 261 - 280 of 856 results