We narrowed to 298 results for: IRES

1. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   Blog Post

   ...switching from paper to an electronic lab notebook requires time and knowledge. In a recent article, we translated...

2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   Blog Post

   ...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...

3. Site Directed Mutagenesis by PCR

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   Blog Post

   ...double-stranded break. This site-directed genome editing requires a template of high homology to the endogenous ...

4. Using Phosphoserine to Study Protein Phosphorylation

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   Blog Post

   ... The generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate...

5. Evolution of Lab Techniques

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   Blog Post

   ...Whereas Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time ...

6. How to Negotiate a Successful Lab Start Up Budget

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   Blog Post

   ...to support your work. For example, if your work requires specialized equipment, try finding institutes ...

7. Antibodies 101: The Basics of Western Blotting

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   Blog Post

   ...overnight.  In contrast, a semi-dry transfer only requires enough buffer to saturate the sandwich and typically...

8. Hot Plasmids: Spring 2025

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   Blog Post

   ...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

9. A Control for All Seasons

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   Blog Post

   ...experiments. A multiplexed experiment, for example, requires staining with multiple primary and secondary antibodies...

10. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial transformation...

11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...membrane, a threshold is reached and the neuron fires, sending the electrical signal down the axon (Lodish...

12. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post

    ...sequence added, leaving a 34 bp “scar”, but currently requires additional experimental manipulation. In some ...

13. Finding Your Science Policy Path

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    Blog Post

    ...and ideas toward solving one problem. It often requires thinking about a problem in a new way through ...

14. A Guide to Getting Started in Undergrad Research

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    Blog Post

    ...assistants), about their experiences. Research requires dedication and effort, so building a strong understanding...

15. Antibodies 101: Validation

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    Blog Post

    ...amenable to high-throughput analyses, however, it requires access to a mass spec facility and the ability...

16. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...auto-fluorescence (Drobizhev et al. 2011). But this requires other lasers than the standard Ti:Sapphire laser...

17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post

    ...another factor to consider. If your application requires longer periods of exposure, you’ll want a more...

18. Antibodies 101: Flow Cytometry

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    Blog Post

    .... It does not, however, permeabilize cells and requires subsequent permeabilization with detergents such...

19. Typing CRISPR Systems

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    Blog Post

    ...systems has led to extreme sequence divergence that requires a multi-pronged analysis to sort effectively. ...

20. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post

    ...weak, random binding to precision gene editing requires three major steps: RNA loading, DNA sequence recognition...