March for Science
Type
Blog Post
...science rallies scientists to political action, and inspires politicians and citizens to include science in...
We narrowed to 298 results for: IRES
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Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations... -
Site Directed Mutagenesis by PCR
TypeBlog Post...double-stranded break. This site-directed genome editing requires a template of high homology to the endogenous ... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post... The generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate... -
Evolution of Lab Techniques
TypeBlog Post...Whereas Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time ... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...to support your work. For example, if your work requires specialized equipment, try finding institutes ... -
Hot Plasmids: Spring 2025
TypeBlog Post...where those interactions occur in the genome. This requires both targeting a specific genome location and ... -
A Control for All Seasons
TypeBlog Post...experiments. A multiplexed experiment, for example, requires staining with multiple primary and secondary antibodies... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...overnight. In contrast, a semi-dry transfer only requires enough buffer to saturate the sandwich and typically... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial transformation... -
Antibodies 101: Validation
TypeBlog Post...amenable to high-throughput analyses, however, it requires access to a mass spec facility and the ability... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...sequence added, leaving a 34 bp “scar”, but currently requires additional experimental manipulation. In some ... -
Finding Your Science Policy Path
TypeBlog Post...and ideas toward solving one problem. It often requires thinking about a problem in a new way through ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...membrane, a threshold is reached and the neuron fires, sending the electrical signal down the axon (Lodish... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...assistants), about their experiences. Research requires dedication and effort, so building a strong understanding... -
Typing CRISPR Systems
TypeBlog Post...systems has led to extreme sequence divergence that requires a multi-pronged analysis to sort effectively. ... -
Antibodies 101: Flow Cytometry
TypeBlog Post.... It does not, however, permeabilize cells and requires subsequent permeabilization with detergents such... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...another factor to consider. If your application requires longer periods of exposure, you’ll want a more... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...auto-fluorescence (Drobizhev et al. 2011). But this requires other lasers than the standard Ti:Sapphire laser... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...