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We narrowed to 771 results for: LIS

Showing: 261 - 280 of 771 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...gene expression (see www.nursa.org for a curated list of coregulators). Coregulators can function as coactivators... regulatory regime (such as genes involved in metabolism, growth or cytokine action). Primary or core ...the NURSA website will feature direct links to listings of available plasmids for our curated NRs and ...

  2. Engaging with science and society at pgEd

    Type
    Blog Post
    ...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai...between these communities and the biomedical establishment. This, along with concerns about the use of ...obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to pgEd as a ...

  3. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids...designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little more complicated. These...the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA from bacteria and..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...

  4. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...team from China’s Ribobio, the illustrious speaker list attracted a host of sponsors based all over the ...one take dried seahorse for?), but there were no English speakers around. Opening talks – Genomics discoveries...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...resistant bacteria. See the picture to the left for his list of the bacterial species that “ESKAPE” antibiotic...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...enrichment via enzyme-linked immunosorbent assay (ELISA) The enriched sybody pools from the phage display...the protein of interest. The Seeger lab used the ELISA technique described in their protocol because it...starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the ...detected using a colorimetric reaction. After the ELISA step, the narrowed-down pool (usually 10-30) of ...

  6. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ... is a list of poster titles or abstracts available before the conference, comb through that list and note... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

  7. Of Myc and Men

    Type
    Blog Post
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still... antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...been an area of intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection... transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated... to perform, often show low efficiency, while biolistic bombardment, which can have high efficiency, requires...

  9. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...’ button a new content-aware suggestion dropdown list will let you jump directly to the type of information...to the plasmid page at the bottom of the dropdown list: The search bar will suggest direct links to other... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...the ‘X’ next to any filter to clear it. Here’s a list of available filters to refine your search results...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the...purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western blots...tags and fusion proteins as examples. The tables list more common tags than are described in the post,...Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification is provided...

  11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins and their mutations...mutations relative to wildtype GFP. Although not listed here, many permutations within each color also exist...plasmids as a means to many functional ends. We’ve listed our favorites below, but many other uses currently...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the best...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized microtubule...transport, and cell division, but given the long list of roles played by microtubules, Opto-katanin will...understand their roles in forming the cilia that help establish left-right organization of the embryo (Aljiboury...the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically controlled phospholipase...

  13. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    ... contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot who has...neutral name. The Addgenies will choose up to four finalists and we’ll ask you to vote for your favorite name...We hope you submit your best ideas because the finalists will receive some newly designed Addgene swag....

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Mammalian Cells Note: This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong...However, proper control experiments are needed to establish the dynamic range of your assay. These experiments...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

  15. Addgene at SfN23!

    Type
    Blog Post
    ...Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working...is rounded out with our science communication specialist (you'll know me by my Tweeting — er, posting!...

  17. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...learn how they think about communicating science. Listen to the interview with Science in the News, Part...Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks to our guests,... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  18. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...This selection should populate your “PubMed filter list” to the left of your screen. That’s it! Next time...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

  19. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...board where he will help shape Addgene's future. Listen to this episode for insights into the process of... tools, and the potential uses of gene drives. Listen here! Podcast breakdown (Time : Topic) 0:00 - 2... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  20. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his research ...opportunities to Ghanaian scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 :... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...
Showing: 261 - 280 of 771 results