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We narrowed to 773 results for: LIS

Showing: 261 - 280 of 773 results
  1. Science Career Options

    Type
    Blog Post
    ... working towards a specific goal, and when you realise that your goal might not lead to a career you actually...available to those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from...really show that you have some of the abilities listed above. Showing that you are willing to exercise...Experiment with New Careers while in Grad School Listen to Biotech Founder Michael Koeris' Journey from...
  2. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...long run.  If you are overwhelmed with your to-do list and your team doesn’t seem to have enough to do…...the entire process is completed more efficiently. Listen to our podcast interview with Harvard Medical School...desired outcomes. Concern yourself with what is accomplished, rather than detailing how the work should be...in more engaged, successful employees who can accomplish great things.   To read more on this topic and...
  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...Outreach Manager for Science Borealis (hyperlink http://scienceborealis.ca/)    ... grant application? Are you familiar with the publishing or patent application processes? How about facilitating...look at the jobs you are interested in. Consider listing the skills you already have, and the skills you...
  4. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...genes across the genome. Although this tool was published in 2011, it was only recently made available through...outer mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of ...shRNA. This system requires less work than using established methods and minimizes some of the technical issues...the CRUSH vector in transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which...Chen and colleagues for genome editing inXenopus tropicalis. (Guo et al., Development. 2014..) A new, higher...plasmids you would like to seen on our Hot Plasmid list. Other popular plasmids & blog posts @Addgene: ...
  5. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...Tools to Try  Checklists Found in surgical suites, air fields, and military bases, checklists are the ultimate...accurately. Thankfully, you should already have a checklist for everything you do in the form of a protocol...protocol a few times, you no longer need the full checklist. Great! But don’t just stop using it cold turkey...
  6. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...containing that enzyme, and embedded in the References lists on every page you can find direct links to plasmids...content and much, much more.     Fig. 2 – List of resources and properties for a Cas entry (SpyCas9a...      CRISPRpedia IGI’s digital resources in English/Spanish  ...
  7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ... vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...of cells affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential...Portugal. She is particularly interested in science journalism and writing about biomedical issues. Follow her...260. PubMed PMID: 16807804. Hanahan, Douglas, and Lisa M. Coussens. "Accessories to the crime: functions...
  8. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. We are blessed with a ...often focus on the excitement of what we are accomplishing and what more we might do. I am grateful every...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...
  9. Working with Nuclear Receptors

    Type
    Blog Post
    ...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...gene expression (see www.nursa.org for a curated list of coregulators). Coregulators can function as coactivators... regulatory regime (such as genes involved in metabolism, growth or cytokine action). Primary or core ...the NURSA website will feature direct links to listings of available plasmids for our curated NRs and ...
  10. Engaging with science and society at pgEd

    Type
    Blog Post
    ...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai...between these communities and the biomedical establishment. This, along with concerns about the use of ...obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to pgEd as a ...
  11. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids...designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little more complicated. These...the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA from bacteria and..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...
  12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...team from China’s Ribobio, the illustrious speaker list attracted a host of sponsors based all over the ...one take dried seahorse for?), but there were no English speakers around. Opening talks – Genomics discoveries...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...resistant bacteria. See the picture to the left for his list of the bacterial species that “ESKAPE” antibiotic...
  13. Tips to Make the Most of a Scientific Conference

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    Blog Post
    ... is a list of poster titles or abstracts available before the conference, comb through that list and note... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...
  14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post
    ...enrichment via enzyme-linked immunosorbent assay (ELISA) The enriched sybody pools from the phage display...the protein of interest. The Seeger lab used the ELISA technique described in their protocol because it...starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the ...detected using a colorimetric reaction. After the ELISA step, the narrowed-down pool (usually 10-30) of ...
  15. Of Myc and Men

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    Blog Post
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still... antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...
  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...been an area of intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection... transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated... to perform, often show low efficiency, while biolistic bombardment, which can have high efficiency, requires...
  17. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...’ button a new content-aware suggestion dropdown list will let you jump directly to the type of information...to the plasmid page at the bottom of the dropdown list: The search bar will suggest direct links to other... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...the ‘X’ next to any filter to clear it. Here’s a list of available filters to refine your search results...
  18. Plasmids 101: Protein tags

    Type
    Blog Post
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the...purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western blots...tags and fusion proteins as examples. The tables list more common tags than are described in the post,...Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification is provided...
Showing: 261 - 280 of 773 results