We narrowed to 417 results for: NUC

...enzyme cloning, TA cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden ... Liu and colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding...

...will include the free nucleotide dGTP in the reaction (exclude all other nucleotides from standard polymerase...without ligation. Because of its dual polymerase/exonuclease functions, T4 DNA polymerase can create overhangs... which a single dNTP is included to stop the exonuclease function of the enzyme and shift its activity...polymerase protocol), causing the enzyme to perform exonuclease activity until the first "G" in the sequence.... the polymerase reaction is favored over the exonuclease reaction, the polymerase will add back the guanosine...manufacturer. It is very important to remove all free nucleotides from your PCR product before proceeding, as they... they will interfere with the exonuclease activity of T4 Pol in the following step. This may be accomplished...

...genome editing of human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created...

...apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, ...

...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...

...Genome Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome...

...Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep 15;8:184-197. PMID: 28918020 AAV6(dbY-F...

...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...also requires two unique single stranded DNA oligonucleotide (oligo) primers, which anneal to the regions...and the hydrogen bonds are broken between the nucleotide base pairs. The initial denaturation step is ...increases the error rate of the DNA polymerase. Deoxynucleoside Triphosphates (dNTPs): Are the building blocks...strands of the template DNA. They are strands of nucleic acid that are starting points for DNA elongation...

...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to ...

...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...etc.) involves selecting one or more unique oligonucleotide primers that flank the regions of your plasmid...colored peaks corresponding to one of the four nucleotide bases. This is an example of a trace file from...

...and DETECTR, two CRISPR-based methods to detect nucleic acids.  On the third day of CRISPR, Addgene gave...

...ssDNA ends from the ssDNA-specific endonuclease activity of T5 Exonuclease and furthermore reduces secondary...specific and unique function for the reaction: T5 Exonuclease - creates single-strand DNA 3’ overhangs by chewing... High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed DNA fragments...

...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino acid...

...standard curve RNase-free DNase 10X DNase buffer Nuclease-free water Microcentrifuge tubes 96-well optical...0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid...10 9 stock or previous dilution (μL) Volume of nuclease-free water (uL) Molecules per μL 10 90 2 x 10 ...Dilution Series Volume of sample (uL) Volume of nuclease free water (uL) Dilution factor Total dilution...

...needs. We may even be able to alter the type of nucleic acid sequences that Cas9 can target." As further...

...for human genome editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b...

...technology, the ability to direct enzymes – from nucleases to transcription factors – to specific sequences...

...cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally...

... 5 minutes to allow for dissociation of the nucleoprotein complexes. The effectiveness of your RNA isolation...agarose gel, or bioanalyzer. For more information on nucleic acid quantification, see our protocol for DNA quantification...