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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 423 results for: NUC

Showing: 261 - 280 of 423 results

  1. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to ...

  2. The Twelve Days of CRISPR

    Type
    Blog Post
    ...and DETECTR, two CRISPR-based methods to detect nucleic acids.  On the third day of CRISPR, Addgene gave...

  3. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...etc.) involves selecting one or more unique oligonucleotide primers that flank the regions of your plasmid...colored peaks corresponding to one of the four nucleotide bases. This is an example of a trace file from...

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...ssDNA ends from the ssDNA-specific endonuclease activity of T5 Exonuclease and furthermore reduces secondary...specific and unique function for the reaction: T5 Exonuclease - creates single-strand DNA 3’ overhangs by chewing... High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed DNA fragments...

  6. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...needs. We may even be able to alter the type of nucleic acid sequences that Cas9 can target." As further...

  7. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...standard curve RNase-free DNase 10X DNase buffer Nuclease-free water Microcentrifuge tubes 96-well optical...0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid...10 9 stock or previous dilution (μL) Volume of nuclease-free water (uL) Molecules per μL 10 90 2 x 10 ...Dilution Series Volume of sample (uL) Volume of nuclease free water (uL) Dilution factor Total dilution...

  10. Let There Be LITE Plasmids

    Type
    Blog Post
    ...cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally...

  11. Editor's Choice, August 2016

    Type
    Blog Post
    ...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ... 5 minutes to allow for dissociation of the nucleoprotein complexes. The effectiveness of your RNA isolation...agarose gel, or bioanalyzer. For more information on nucleic acid quantification, see our protocol for DNA quantification...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity to the “dopamine...
Showing: 261 - 280 of 423 results