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1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   ...extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent proteins with this...

2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...specific plasmids. This method, therefore, reduces the number of plasmids that are required in the transfection...

3. GCE4All: Making Genetic Code Expansion Accessible

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   ...specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The rapid expansion...

4. Improved Plasmid Maps Powered by SnapGene

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   ... enzymes will cut (in basepairs), and the total number of times they cut. Use the filters on the right...

5. Luminescent Imaging with Nano-lanterns

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   ...high luminescent signal for imaging of low-copy number proteins, whereas such detection is difficult using...

6. Multiplex Genome Editing with CRISPR-Cpf1

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   ...variable expression levels due to differences in copy number.  And there are the usual transfection drawbacks...

7. Screening for Successful Genome Editing with Digital PCR

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   ...statistical modelling can be used to determine the actual number of target DNA molecules present in the original...

8. Addgene’s Viral Service - Why Virus? Why Now?

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   ...we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...

9. Top Requested Lentivirus and AAV of 2016

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   ... found the most useful so far? We crunched the numbers on our Viral Service (and then we crunched them...

10. Antibodies 101: Monoclonal Antibodies

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    ...hybridoma impacts the specificity, avidity, and number of different antibodies produced by the hybridoma...

11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...expressing cells exhibited a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted...

12. A Novice's Perspective on Neuroscience - SfN2017

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    ...neuroscience and, like many biological fields the number potential of experiments enabled by physical, software...

13. Why and how to start studying chromatin (with Addgene's help)

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    ...transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how...

14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...biology, cloning a gene into your plasmid is often a numbers game. By using a variety of screening and selection...

15. Hot Plasmids - May 2022

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    ...due to multiple factors, including limitations of number of flourescent proteins that can be inserted into...

16. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ...overexpression and knockdown. Addgene also has a number of insect vectors to consider. An easy way to see...

17. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited and asked for the protocol...

18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...considering the gRNA itself is ~100 bases) limit the number of gRNAs that can be packaged together in a single...

19. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ... said that there is no theoretical limit on the number of genes that can be manipulated at one time. Weissman...

20. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    ... we going to talk about?” There are an infinite number of ideas on the internet, but start by downloading...