We narrowed to 416 results for: Numb

1. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   ...neuroscience and, like many biological fields the number potential of experiments enabled by physical, software...

2. Hot Plasmids - May 2022

   Type

   Blog Post

   ...due to multiple factors, including limitations of number of flourescent proteins that can be inserted into...

3. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   ...hybridoma impacts the specificity, avidity, and number of different antibodies produced by the hybridoma...

4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...specific plasmids. This method, therefore, reduces the number of plasmids that are required in the transfection...

5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   ...overexpression and knockdown. Addgene also has a number of insect vectors to consider. An easy way to see...

6. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   ... enzymes will cut (in basepairs), and the total number of times they cut. Use the filters on the right...

7. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   ...high luminescent signal for imaging of low-copy number proteins, whereas such detection is difficult using...

8. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   ...variable expression levels due to differences in copy number.  And there are the usual transfection drawbacks...

9. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...statistical modelling can be used to determine the actual number of target DNA molecules present in the original...

10. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    ...we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...

11. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    ... found the most useful so far? We crunched the numbers on our Viral Service (and then we crunched them...

12. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    ...specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The rapid expansion...

13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ...ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited and asked for the protocol...

14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    ...considering the gRNA itself is ~100 bases) limit the number of gRNAs that can be packaged together in a single...

15. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    ... said that there is no theoretical limit on the number of genes that can be manipulated at one time. Weissman...

16. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ... we going to talk about?” There are an infinite number of ideas on the internet, but start by downloading...

17. Protocol - How to Inoculate a Bacterial Culture

    Type

    Protocol

    ...present in large numbers, 50 or more per cell and have a high copy number. High copy number plasmids should...copy and low copy plasmids? The copy number refers to the number of copies of an individual plasmid within... of bacteria and is used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid...bacterial cell. Large plasmids usually have a low copy number (approximately one or two copies per cell) and ...

18. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...denoted using two numbers separated by a slash (e.g. AAV2/5), where the first number indicates the serotype...far been identified, and it is likely that this number will continue to increase. This diversity makes...serotype of the genome and the second number the serotype of the capsid. Pseudotyped vectors can improve... BBB. The Viviana Gradinaru lab has developed a number of systemic rAAV vectors that can target whole ...

19. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    ...scientists within and outside your field, and any number of interactions with professionals from other backgrounds...

20. dTAG - You're it!

    Type

    Blog Post

    ...will provide an update with vendor and catalog numbers. As we are happy to share aliquots of dTAG-13 in...