We narrowed to 538 results for: PAN

1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   Blog Post

   ...exciting to see that the use of optogenetic tools is expanding to immunological research. Here the Zhou group...excitable but responsive to calcium. We’d love to expand our cancer and immunology resources. If you have...

2. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...Improving Nano-lanterns In 2015, Takai et al. expanded the Nano-lantern color palette to include cyan... this paper at Addgene. Takai, Akira, et al. “Expanded palette of Nano-lanterns for real-time multicolor...

3. CasPEDIA: A Functional Classification of Cas Enzymes

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   Blog Post

   ...Cas9 The Other Cas(s) The PAM Requirement and Expanding CRISPR Beyond SpCas9 More resources from the ...CRISPRpedia IGI’s digital resources in English/Spanish  ...

4. Viral Vectors 101: Biosensors

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   Blog Post

   ...sensors exist and are ready-to-use. While biosensors span the gamut of applications within chemistry, biology...cell culture models. Some of them have even been expanded for multiplexed imaging for advanced monitoring...

5. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...offered domestically, but by the end of 2016, we expanded our viral inventory to 25 lentiviruses and 25 ...initial success, we will continue to provide and expand this diverse and useful collection of tools so ...

6. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   Blog Post

   ...Susan. After coming to this realization, Susan panicked. She had always loved science, but didn’t know...science communication] needs. I worked for one company, but there were a ton of others that also did work...

7. Seven Tips for Using LinkedIn as a Scientist

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   Blog Post

   ...option is in the “More…” menu. You can also follow companies and organizations to see their news and job postings...interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include news, inclusion...

8. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...source (i.e. primer3) or provided by the major companies selling PCR reagents. Nowadays commercial polymerases...can use the chromatogram to spot whether any discrepancies between your sequencing result and the expected...

9. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   Blog Post

   ...Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research. But... Schwessinger joined this cause and helped by expanding this team, leading monthly calls, and planning...

10. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ...accomplished by using a panel of primary antibodies conjugated to different fluorophores or a panel of primaries ...

11. What's New in CRISPR - November 2021

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    Blog Post

    ...gene transfer with pseudotyped viral particles, expanding options for CRISPR or other gene delivery. Read...performance at therapeutically relevant gene targets and expand the application to the possible treatment of genetic...

12. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ...proportion were found on the plasma membrane (top panel in Fig. 1). Of course, this was problematic, because...hM4Di in plasma membranes in both species (bottom panel in Fig. 1). Our results indicate that the tag protein...

13. Working with Nuclear Receptors

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    ...which will feature a simplified user interface, expanded numbers of curated molecules, including 400 or...or so coregulators, and a greatly expanded set of links to datasets and reagents in the field. Additional...

14. Rabies and Neuronal Tracing

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    Blog Post

    ... neurons integrated into the brain over time (Deshpande et al., 2013). The study found that at early time...Dec; 18(6): 617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, Lepier ...

15. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post

    ...worm amputations. They also created an online companion resource called “cutting class” which provides...encourage you to deposit them back with Addgene and expand the number of genes that other classrooms and researchers...

16. Hot Plasmids and Viral Preps - January 2021

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    ... Yongsub Kim’s lab engineered prime editors to expand their PAM flexibility. Find the engineered prime...some of the new viral preps from recent months: Expanded serotypes are now available for Gordon Fishell...

17. Corralling and Cornering the Goldilocks Antibody

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    ...-mount IHC techniques on rodent brains and is expanding to more complex brain systems such as non-human...monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of ...

18. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes...Central PMCID: PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle:...

19. CRISPR in the Clinic

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    Blog Post

    ... functional correction of the gene in clonally expanded organoids. This work, and many others, pushed ...for use in the next few months. Several other companies have followed suite, with Beam Therapeutics initiating...

20. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post

    ...the data-driven approach was needed to massively expand complexity. The three tools developed, outlined...specific overhang sequences, allowing it to be used to expand already-existing sets. Note that this tool relies...