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We narrowed to 653 results for: PPL

Showing: 261 - 280 of 653 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...missing or added at the broken ends and, as such, cripple the gene. However, if repair material is provided... with Biogen and is particularly interested in applying CRISPR for mouse genome editing.     Haoyi Wang...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge to help researchers identify...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    ... be mostly on the brain, but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts... infect) can influence an AAV’s value greatly. Applying this mantra to AAV selection, there are three ...only can we compare these methods in specific applications, we may also be able to use these comparisons...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...silencing can be easily induced by exogenous application of long or short dsRNA. However, it is important...have benefited from the ease of use in dsRNA applications. Figure 1: Schematic of an RNAi-inducing..., which is conserved in many organisms. When supplemented with adenine analogs such as 2-fluoroadenine...

  7. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... have been completed. Therefore, to be ready to apply for a Group Leader position, key first author manuscripts... project. Furthermore, Group Leader position applications require 3 letters of reference- the graduate...informal recommendations, more positive grant application and manuscript reviews, etc. You'll also meet...

  8. Typing CRISPR Systems

    Type
    Blog Post
    ...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://...Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

  9. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  11. We’re Going Viral This Week!

    Type
    Blog Post
    ... information on the basics of viral vectors, applications, and some new resources available from Addgene... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

  12. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ... a lab supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament...

  13. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

  14. CRISPR Cheat Sheet

    Type
    Blog Post
    ...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...

  15. Summer Fun at Addgene!

    Type
    Blog Post
    ... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

  17. Editor's Choice, July 2016

    Type
    Blog Post
    ...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...this post to anyone wondering if and how they can apply FRET in their own research. All posts from July ...

  19. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic...

  20. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...cellular imaging with ultrasound. In his research he applies naturally occurring bacterial “molecular buoyancy...biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows dye visualization...
Showing: 261 - 280 of 653 results