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We narrowed to 651 results for: PPL

Showing: 261 - 280 of 651 results

  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to ...mutations are introduced, and scalability (see Supplementary Table 1 of Wrenbeck et al). It uses a uracil...about the same number of positive clones. See Supplementary Note 1 in Wrenbeck et al for details on how ...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    ...comparison to a control sample. Southern blotting applications range from confirming the results of cloning...sequence thousands of reads at the same time. Other applications of sequencing, such as confirming the results...replaced these, these techniques still have useful applications and have informed many current techniques. For...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...success in basic research, researchers are eager to apply it in a clinical setting. CRISPR is often used for...estimated that exon skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon...researchers are very excited. This approach may also be applicable to a number of rare genetic diseases caused by...

  4. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  5. A Control for All Seasons

    Type
    Blog Post
    ...secondary antibodies.   Application-specific controls Some antibody-based applications have very specific ...then moving on to controls for antibody-related applications. After reading, you will have a better understanding...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    .... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...Retrograde Serotype Viral Vectors 101: Viral Applications...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...multiplexed IF, many of the same considerations apply as for standard IF. However, there are a few specific...primary antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies...Immunocytochemistry Protocols for other antibody applications...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

  9. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...missing or added at the broken ends and, as such, cripple the gene. However, if repair material is provided... with Biogen and is particularly interested in applying CRISPR for mouse genome editing.     Haoyi Wang...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge to help researchers identify...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...silencing can be easily induced by exogenous application of long or short dsRNA. However, it is important...have benefited from the ease of use in dsRNA applications. Figure 1: Schematic of an RNAi-inducing..., which is conserved in many organisms. When supplemented with adenine analogs such as 2-fluoroadenine...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... have been completed. Therefore, to be ready to apply for a Group Leader position, key first author manuscripts... project. Furthermore, Group Leader position applications require 3 letters of reference- the graduate...informal recommendations, more positive grant application and manuscript reviews, etc. You'll also meet...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ... be mostly on the brain, but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts... infect) can influence an AAV’s value greatly. Applying this mantra to AAV selection, there are three ...only can we compare these methods in specific applications, we may also be able to use these comparisons...

  14. Typing CRISPR Systems

    Type
    Blog Post
    ...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://...Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

  15. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  17. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ... a lab supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament...

  18. We’re Going Viral This Week!

    Type
    Blog Post
    ... information on the basics of viral vectors, applications, and some new resources available from Addgene... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

  19. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

  20. Summer Fun at Addgene!

    Type
    Blog Post
    ... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...
Showing: 261 - 280 of 651 results