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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 601 results for: RET

Showing: 261 - 280 of 601 results

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...proteins were successfully expressed as fusions that retain their individual activities (1). Researchers also...DBD) and transcriptional activation (TA) domains retain their individual activities when separated (2),...

  4. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...cell’s genome, so no special sequence is needed to retain the vector as cells divide. In transient transfection... and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular...

  5. How to Be an Excellent Trainee

    Type
    Blog Post
    ...trainee (even if you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee...gives twofold benefits: you may learn faster and retain more information plus your mentor will get you ...

  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...in human pluripotent stem cells(hPSCs), Lindy Barrett’s lab at the Broad Institute developed fluorescently...

  8. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...known 'DNA adenosine deaminases' which would theoretically give rise to an adenine base editor. A massive...developed using the hAPOBEC3 deaminase can edit in a stretched window ranging from 1-17 bp relative to the PAM...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...antibodies. In contrast, with their many epitopes, smFPs retain their ability to be detected after sample preparation...the Addgene Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus ...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...typically contains one viral genome. Other than a theoretical maximum value, physical titers don’t necessarily...different sources in the same experiment, consider retitering both AAV in your lab. The absolute titer values...

  12. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...faculty, students, private sector researchers, or pretty much anyone applying the scientific method to a..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

  13. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ..., A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology...conditions on the production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development...

  14. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

  15. Optogenetics AAV Preps

    Type
    Collection
    ... Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...%}   Interfering in the future While RNAi has retreated a bit to the background, it remains a solid option...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ..., you’ll first need to learn a little bit of theoretical background on the different parameters that your... tumor sample You made it! So much about the theoretical background. Let’s dive into the praxis of gating...

  18. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...researchers realized they needed specific tools to interpret the roles of individual integrins. The Ha lab ...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...This means all newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in...invasive strand is displaced from the template and returned to the processed end of the non-invading strand...
Showing: 261 - 280 of 601 results