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We narrowed to 741 results for: REV

Showing: 261 - 280 of 741 results

  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...species and applications. In this blog, we will review the most common tags, the antibodies used to detect...optimizations and specific monoclonal antibodies. Below we review the most commonly available tags and their antibodies...Myc was developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit...

  2. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments...to do is co-transform pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter...confined experiments as well as those requiring reversibility that you cannot do with AraC,” says Di Ventura...

  3. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...standard AAV QC regimen does not address this.  Previous studies have demonstrated that NGS is an effective...tagmentation preparation approaches described previously require a preliminary double stranding step. ...specific signature sequences in the data. Unlike previously described thermodynamics-based methods of serotype...

  4. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily transferrable...oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and... of this to control a metabolic network in S. cerevisiae by using three RNA programs to differentially...

  5. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...with the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled...problem was I knew I had a knowledge gap. I had no previous business, marketing or sales training and knew...

  6. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned...fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP...PMCID: PMC3790201. Elmore, Susan. "Apoptosis: a review of programmed cell death." Toxicologic pathology...

  7. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ... is conserved in all living organisms and has previously been used for multiplex Cas9-nuclease editing...existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream lengthy..._VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...Using this road map, and what was known about previously characterized TFs, the Zhang group were able ...most immediately useful for TF library screens. Previously engineered TF libraries were not as comprehensive...transformation and amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications...

  9. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...introductions to potential publications or prepare a review article. These are both options that can be done... started early on introductions and literature reviews could make the months leading up to your defense...Consider submitting a registered report, where peer review is based on the methods, not the results. Also ...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...scientists Anyone can become a member of BosLab and previous laboratory experience is not required. Our members...microbiome project. This project was inspired by previous city-wide genomic studies and our study will involve...

  11. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...educational and great for lab life. These apps prevent you from constantly searching the interwebs for...important biological information, amino acid abbreviations, and structures into one convenient resource...say that I still have the single and triple abbreviations of the amino acids memorized, but the structures...

  12. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...altered by blue light illumination. This ability to reversibly bind and release a protein of interest in response...allows for tagless protein purification as well as reversibly regulation of endogenous signaling activity in...while cycling between dark and blue light which revealed a blue light-dependent shift in mCherry fluorescent...

  14. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...strategy that works. But, those darn cancers have revealed many tricks up their sleeves and most patients...experiments. But, there have been two huge challenges preventing us from testing this hypothesis: There was no... this publication We also wrote an extensive review on the exciting new field we call “precision functional...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...internship. Lastly, have your resume and cover letter revised by your network, which includes your friends, family...personality leap out of the page. Finally, as previously mentioned, enlist the help of your network to...week before the deadline. The day of submission, review your application with a fine comb and hit submit...

  17. Antibodies 101: ChIP

    Type
    Blog Post
    ...formaldehyde or UV light to create strong but reversible bonds. This will keep the protein:DNA complexes...washing and then incubate with Proteinase K to reverse the crosslinking. Finally, purify your DNA with...Shengliu via Wikicommons.   Okay, let’s do a quick review. At this point you have (1) crosslinked your proteins...

  18. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...approach, your workflow, or your physical setup - to prevent the mistake, just like you would change variables...reagents from left to right as they use them.    Prevent Interruptions If you need to focus, a large set...Down At the suggestion of a postdoc, to whom I am forever grateful, I started setting aside 3-5 minutes to...

  19. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...wiki format and includes biochemical properties, previously established uses, and intended applications. ...resources and information For some researchers, the previous information may be all you need to start your ... burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https://doi.org/10.1038...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...display. Pol, the last component, contains the reverse transcriptase and integrase components to convert...relevant viral genes (including the ones listed previously). E3/E1B suppresses both apoptosis and the host...matter to adenovirus production tips. For quick review, all of the elements discussed are compiled for...
Showing: 261 - 280 of 741 results