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We narrowed to 652 results for: SIM

Showing: 261 - 280 of 652 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...ladders are not next to empty wells. Most people will simply load an extra ladder or technical replicate (ie... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...blocking time! Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting...

  2. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...

  5. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... it to fruition. The concept, though relatively simple, changed the course of scientific research. What...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...cerevisiae and S. pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying...ensure that all desired tags have been integrated. Simply design your amplification primers with the desired...

  7. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is a very common...

  13. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...

  14. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...

  16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

  17. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid depositors, ...this resource for the community. There are a few simple steps you can take to input your data for sharing...

  18. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...target protein? Epitope Availability The answer is simple: epitope availability. An antibody can only bind...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

  19. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously developed..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing...
Showing: 261 - 280 of 652 results