We narrowed to 466 results for: Sele

1. Hot Plasmids - February 2022

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   Blog Post

   ...minimizing off-target effects by identifying guide selection rules, and reducing recombination by finding acceptable...

2. Antibodies 101: Buffers, Storage, and Conjugates

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   Blog Post

   ...complicated, but hopefully this helps you successfully select - and keep! - antibodies that are a good match ...

3. Screening for Successful Genome Editing with Digital PCR

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   ...genome-edited human iPS cells without antibiotic selection. Nat Meth 11, 291–293 (2014). PubMed PMID: 24509632...

4. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...efficiency is high enough to negate the need for selection usually required in recombineering. REPLACR-mutagenesis...

5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...mutating and deactivating a gene or genes to selectively activating them with the CRISPR system was a ...

6. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...innovation in plant synthetic biology. In our case, we selected a project in a field with 30 years of basic research...

7. CRISPR Plasmids - Prime Edit

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   ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI...Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9...Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6...

8. Antibodies 101: Conjugation

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   Blog Post

   ...antibody yourself allows you to consider a broader selection of antibodies for your experiments.  Many of ...

9. RNA Extraction Without A Kit

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   Blog Post

   ...the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA and proteins. Lastly...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and...

11. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...mutated it. Using a robotic picking approach to select cells with variant characteristics they wanted,...

12. Summer SciComm: Preprints

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    Blog Post

    ... P, Peters I (2021) Motivations, concerns and selection biases when posting preprints: a survey of bioRxiv...

13. Antibodies 101: Secondary Antibodies

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    ...secondary antibody needs careful consideration before selection. If all the primary antibodies used are mouse ...

14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...sequences. Thirty of these remaining sequences were selected for characterization with patch clamp electrophysiology...

15. Human Germline Editing Using CRISPR

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    Blog Post

    ...cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all...

16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...inactive form unless autophagy is induced. Then non-selective macroautophagy of the cytosol leads to accumulation...

17. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...co-opted to control gene expression, he could then selectively manipulate only GFP-labeled cells. Once Tang ...

18. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...resistant to drug treatment and that these are selected for during the treatment process. The authors ...

19. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post

    ...eligibility information, submission information, selection criteria, and award administration information...

20. Tetracycline Inducible Expression

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    ...expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393...miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional selection options third-generation...for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article...luciferase in cloning site; see article for additional selection and FP option rtTA On Kowarz 16623 pBI-GFP Expression..., and Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains an insert (...