We narrowed to 472 results for: Sele

1. GCE4All: Making Genetic Code Expansion Accessible

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   ...almost endless possibilities for researchers, from selective protein ligation through introducing ncAAs with...

2. Viral Vectors 101: Viral Applications

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   ...constitutive), your gene of interest, and often a selectable marker. This vector can express your construct...

3. Hot Plasmids - February 2022

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   ...minimizing off-target effects by identifying guide selection rules, and reducing recombination by finding acceptable...

4. Antibodies 101: Buffers, Storage, and Conjugates

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   ...complicated, but hopefully this helps you successfully select - and keep! - antibodies that are a good match ...

5. Antibodies 101: Isotypes

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   ...useful to know the isotype of an antibody so you can select an appropriate isotype control. An isotype control...

6. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ... samples arrive, each group of plates will have select samples prepared and screened by NGS to spot check...

7. Screening for Successful Genome Editing with Digital PCR

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   ...genome-edited human iPS cells without antibiotic selection. Nat Meth 11, 291–293 (2014). PubMed PMID: 24509632...

8. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...binding screens, peptide arrays, and phage display selections. In addition to these constructs, the Sidhu lab...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...efficiency is high enough to negate the need for selection usually required in recombineering. REPLACR-mutagenesis...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...mutating and deactivating a gene or genes to selectively activating them with the CRISPR system was a ...

11. 3 Challenges in Plant Synthetic Biology

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    ...innovation in plant synthetic biology. In our case, we selected a project in a field with 30 years of basic research...

12. Antibodies 101: Secondary Antibodies

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    ...secondary antibody needs careful consideration before selection. If all the primary antibodies used are mouse ...

13. RNA Extraction Without A Kit

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    ...the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA and proteins. Lastly...

14. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and...

15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ...inactive form unless autophagy is induced. Then non-selective macroautophagy of the cytosol leads to accumulation...

16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...mutated it. Using a robotic picking approach to select cells with variant characteristics they wanted,...

17. Human Germline Editing Using CRISPR

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    ...cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all...

18. Antibodies 101: Conjugation

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    ...antibody yourself allows you to consider a broader selection of antibodies for your experiments.  Many of ...

19. Summer SciComm: Preprints

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    ... P, Peters I (2021) Motivations, concerns and selection biases when posting preprints: a survey of bioRxiv...

20. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...sequences. Thirty of these remaining sequences were selected for characterization with patch clamp electrophysiology...