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Addgene

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Showing: 261 - 280 of 612 results

  3. Science Career Options

    Type
    Blog Post
    ... all the boxes’. Once you’ve assessed your skill set and done your research, it’s time to start sending... a project, overcoming inevitable failures and setbacks. There are many roles that actively require these...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...polymerase using your favorite PCR protocol.  2. Set Up TOPO Cloning Reaction: Mix together the PCR product...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

  5. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...must be evaluated on a case-by-case basis. While setting up their CPP system to study protein transduction...

  7. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...then the chance is that you have handled large data sets and are familiar with statistical analysis. If you... If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers usually don't...

  9. Hot Plasmids - January 2023

    Type
    Blog Post
    ... Yingxiao Wang lab.  The crux of the system is a set of heat-inducible lentiviral CAR constructs, the ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that contain two hairpin sequences..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...

  12. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase...

  16. How to Lead a Great Meeting

    Type
    Blog Post
    ...for team meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 ...

  19. Brain Initiative Collection

    Type
    Collection
    ...research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository....

  20. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...that is, an antibody shown to perform well in one set of conditions may not perform as well in other conditions...cell types of interest. By correlating your data sets, you can increase confidence that the labeling observed...available. Various proteomic and transcriptomic datasets can be used to select a candidate cell line (e.g...
Showing: 261 - 280 of 612 results