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TypeBlog Post...easily achieved with alternative methods. Ready, set, transduce! Making virus can sound daunting, but ...
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An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post... no idea where to start. Thankfully, my managers set aside time for me to complete an online social media... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered and ... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...percentage of the recombined vector backbone. Ready, set, grow! Our 5 Tips for Troubleshooting Viral Transductions... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...establish that schedule. There’s also tips for setting writing goals, tracking progress, and starting ... -
Another Pathway into Cells: iTOP
TypeBlog Post...must be evaluated on a case-by-case basis. While setting up their CPP system to study protein transduction... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...with a different dCas9 binding module, alternative sets of transcriptional programs can be layered with ... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...then the chance is that you have handled large data sets and are familiar with statistical analysis. If you... If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers usually don't... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post... and detailed protocols on topics such as how to set up your digests and pouring/running a DNA gel. ...glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a visual reference... -
Brain Initiative Collection
TypeCollection...research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository.... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post... to create a fully controllable and reproducible set up,” Bernach explains. Developing an artificial leaf... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that contain two hairpin sequences..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...and plasmids to researchers in resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid... -
Hot Plasmids - January 2023
TypeBlog Post... Yingxiao Wang lab. The crux of the system is a set of heat-inducible lentiviral CAR constructs, the ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository... -
DAP arrays for multiplex gene editing
TypeBlog Post... base editing and prime editing. How do you then set up an MBE system that contains a double-win for both... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...template for PCR amplification with a degenerate primer set to amplify IgG variable light (VL) and variable heavy... -
How to Lead a Great Meeting
TypeBlog Post...for team meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog Post...and the CARE guidelines for case reports are two sets of guidelines frequently used by editors. Providing... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...series will cover 2 of the 7 mentoring questions I set out to answer. First, I will describe some of the...