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  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...polymerase using your favorite PCR protocol.  2. Set Up TOPO Cloning Reaction: Mix together the PCR product...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

  5. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...must be evaluated on a case-by-case basis. While setting up their CPP system to study protein transduction...

  7. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...then the chance is that you have handled large data sets and are familiar with statistical analysis. If you... If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers usually don't...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that contain two hairpin sequences..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase...

  15. Hot Plasmids - January 2023

    Type
    Blog Post
    ... Yingxiao Wang lab.  The crux of the system is a set of heat-inducible lentiviral CAR constructs, the ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

  16. How to Lead a Great Meeting

    Type
    Blog Post
    ...for team meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 ...

  19. Brain Initiative Collection

    Type
    Collection
    ...research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository....
Showing: 261 - 280 of 609 results