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...beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... a high multiplicity of infection, because this setup allows for experimental replicates for each of the...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

...Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https...vector genomes as containing just the expression cassette that we want, it also includes a small amount ...

...Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer design...

...other epitopes and scaffolds to build a more robust set of frankenbodies. References Ewert, S., Honegger...

...first 3 books of a larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost and...

...long email. If you have more to say, email again or set up a meeting. If you really got along well, suggest...

...polymerase using your favorite PCR protocol.  2. Set Up TOPO Cloning Reaction: Mix together the PCR product...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

...must be evaluated on a case-by-case basis. While setting up their CPP system to study protein transduction...

...with a different dCas9 binding module, alternative sets of transcriptional programs can be layered with ...

...then the chance is that you have handled large data sets and are familiar with statistical analysis. If you... If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers usually don't...

... and detailed protocols on topics such as how to set up your digests and pouring/running a DNA gel.  ...glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a visual reference...

... Yingxiao Wang lab.  The crux of the system is a set of heat-inducible lentiviral CAR constructs, the ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

... base editing and prime editing. How do you then set up an MBE system that contains a double-win for both...

...template for PCR amplification with a degenerate primer set to amplify IgG variable light (VL) and variable heavy...

... to create a fully controllable and reproducible set up,” Bernach explains. Developing an artificial leaf...

...they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that contain two hairpin sequences..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...

...mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase...

...and plasmids to researchers in resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid...

...for team meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 ...

...and the CARE guidelines for case reports are two sets of guidelines frequently used by editors. Providing...