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... appear within a range of wavelengths. For that reason, we speak of excitation and emission spectra. Both... happens due to a process called Fluorescence Resonance Energy Transfer (FRET).   Figure 1: Emission...fused to CD45, but is separately expressed. (The reason for having a separately expressed transduction ...difficult. I chose BV421 for the following two reasons: 1) The emission spectrum of BV421 is far enough...

... work, funding for science, and numerous other reasons. It is important to remember that we, scientists...Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson Over the last few years, there's been a disturbing...positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear Science and...enforcement, and, although there was at least one person vocally protesting us, he was not threatening in...

...Many thanks to our guest blogger, Doug Richardson! Doug Richardson is the Director of the Harvard Center...This post was contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and... 2, 932-940 (2005). PubMed PMID: 16299478. 9. Richardson DS, Lichtman JW. Clarifying Tissue Clearing. ...

... something is working for you, then there is no reason to change it. But what if a kit isn’t working for...variety of manufacturers. It also includes a rough comparison of list price between these manufacturers, as...kits before deciding which one to buy. Table 3: Comparison of popular plasmid purification kit options ...blog post was written by Ashley Waldron, Hannah Dotson, and Nyla Naim. Resources Additional resources...

...for near-freezing temperatures, our first of the season snow flurries, dusting off the winter coats and... to #DeckTheLab with virus snowflakes from @CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make...WFH space feel a little more festive this holiday season! It is that time of the year again: miniature ...

...Serotype Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics...Biosensors Li New viral service pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

...PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype... Rachel Leeson...

...vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024), developed and deposited at Addgene.... Figure used with permission from Milling and Markson et al., 2024.   Proof of concept The FITS approach...cancer.   References and resources Milling, L. E., Markson, S. C., Tjokrosurjo, Q., Derosia, N. M., Streeter...

...description of the tasks I would be responsible for, in comparison to the other positions listed with a much less...flexibility was the ability to work either remotely or in person. This was useful to me as some days it was not ...their employees are feeling comfortable.     Lucie Wilson is a rising junior and bioengineering student at...

...note that these results could be biased for two reasons: 1) they are based on the US Occupational Employment...nonscientists, blur the line between professional and personal on Twitter. Or maybe scientist prefer to share...Tips: Online Social Networking for Scientists 5 Reasons to Use Reddit for Science Communication 10 Ways...

...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer...heterotrimeric G protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the...

...depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool (CsChrimson) attached to a fluorescent...Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin TR (2019) SPARC: a method to genetically...

...mosaic virus Dr. Fiona Robertson, University of Zimbabwe, Zimbabwe Dr. Fiona Robertson (far left) is a Senior...potential for this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology...

...scientific community, what other reasons do we like being a nonprofit? One big reason is that scientists and technology..., Eric, Gizela, Henry, Ina, Inna, Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, ...

...primers for In-Fusion Cloning Design primers for Gibson Assembly and NEB HiFi DNA Assembly  OligoAnalyzer...cloning methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion cloning.  NEB Ligation ...resources were added in September 2023 by Rachel Leeson.  Additional resources on the Addgene blog: ...

...matters! There are important societal reasons, self-interested reasons, and, given that science is largely...largely funded by tax-payers, there are reasons to feel obligated too, as experts on the subject brought out...

... mind from that session were words from Eric Hendrickson, loosely paraphrased in the tweet below. The... DNA for Enhanced CRISPR Genome Editing Chris Richardson and members of Jacob Corn’s lab describe how ...Yinan, Brian Ruis, Taylor Takasugi, and Eric A. Hendrickson. Genome Res 27(7) (2017):1099-1111. PubMed PMID...

... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://...

...AAV9  pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 124603  AAV9  pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-...affect DREADD trafficking and localization. The Sternson lab recently deposited PSAM AAV, which encode ...

...visualization tools using CRISPR In 2015, Thoru Pederson’s lab at the University of Massachusetts Medical...CRISPR-Sirius Recently, Hanhui Ma and the Thoru Pederson lab developed CRISPR-Sirius (Ma et al., 2018)....order to label two loci simultaneously, Ma and Pederson created another sgRNA containing the PP7 hairpin...