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We narrowed to 729 results for: Son

Showing: 261 - 280 of 729 results

  1. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer...heterotrimeric G protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the...

  2. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...matters! There are important societal reasons, self-interested reasons, and, given that science is largely...largely funded by tax-payers, there are reasons to feel obligated too, as experts on the subject brought out...

  3. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ... mind from that session were words from Eric Hendrickson, loosely paraphrased in the tweet below. The... DNA for Enhanced CRISPR Genome Editing Chris Richardson and members of Jacob Corn’s lab describe how ...Yinan, Brian Ruis, Taylor Takasugi, and Eric A. Hendrickson. Genome Res 27(7) (2017):1099-1111. PubMed PMID...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...AAV9  pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 124603  AAV9  pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-...affect DREADD trafficking and localization. The Sternson lab recently deposited PSAM AAV, which encode ...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...visualization tools using CRISPR In 2015, Thoru Pederson’s lab at the University of Massachusetts Medical...CRISPR-Sirius Recently, Hanhui Ma and the Thoru Pederson lab developed CRISPR-Sirius (Ma et al., 2018)....order to label two loci simultaneously, Ma and Pederson created another sgRNA containing the PP7 hairpin...

  6. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...science trainee will spend 4-7 years working for one person and with a group who all strive for a common big-picture...rotation, which reveals their working habits, personality, and gives them the opportunity to see if the...extreme openness” of the lab (i.e projects like the Personal Genome Project and open source software, hardware...

  7. Cloning

    Type
    Guide
    ...back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type...Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning Oligo Stitching...cost, availability of starting materials or just personal preference. Check out our blog on choosing the... is the "classic" cloning method, and for many reasons, remains one of the most popular today. Restriction...Plasmid 101 blog post on TOPO cloning. Back to Top Gibson Assembly (Isothermal Assembly Reaction) Isothermal...Isothermal cloning, more commonly known as Gibson assembly ( protocol ), takes advantage of the properties ...lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments with 20-40 base pair homology...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different variants ...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available at ...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...1kb fragments are produced (see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: They are comprised...

  9. Hot Plasmids - May 2022

    Type
    Blog Post
    ...error rate for long sequence knock-ins by: Lucie Wilson Le Cong’s lab has developed a method using dCas9...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...barcodes for live cell clonal multiplexing by: Rachel Leeson Multiplexed images in live cells remains a challenge...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    ... variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes when...0600-6. The Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system...mammalian genome regulation and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion...

  11. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ...JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal ...

  12. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ... proto-oncogenes and disrupt their expression (Robinson, 1982). To add insult to injury, retroviruses ...immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer. Rev Infect Dis. 1982...Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI...

  13. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ... (fluorescent and luminescent) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy...which then emits it as light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except...tips below! This post was originally written by Jason Niehaus in June 2014 and was updated by Emily P....

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... Cpf1 for multiplex genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple...guide page for a review of Cas9.  For a brief comparison of Cpf1 vs. Cas9, see the table below. Table...expression vector.  See the graph in figure 2 for a comparison of editing frequency resulting from transfection...

  15. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ... the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty ...the function of the protein. Myers: We’ve done comparisons of our new system versus standard ChIP-seq for... for transcription factors, but there are many reasons you might want a system like this. It’s actually...

  16. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E...P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson EA, Ramsden DA (2014) The fidelity of the ligation...

  17. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...want to cover during the year. Suggest that each person bring 3 topics and write them on a board to see... and meet regularly no matter what, even if one person can’t make it. One of the biggest group mentoring...meetings. Experiment with the process. Have each person write a Development Plan. Set concrete goals with...

  18. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... on what makes them that way. Is there a senior person whose career path you are interested in following.... Finding potential mentors is one of the many reasons to always be growing your network. If you don’t...need for your current position Understand your interpersonal challenges if they know some of the people you...

  19. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...decades to generate knock-out mice, but for various reasons it is not nearly as efficient in human stem cells...genetic background and do an apples-to-apples comparison of all those mutations. If you make induced pluripotent...backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR...
Showing: 261 - 280 of 729 results