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We narrowed to 564 results for: Vit;

Showing: 261 - 280 of 564 results
  1. New and Upcoming Viral Vectors - September 2019

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    Blog Post
    ... continue to be useful for monitoring neuronal activity in the form of calcium dynamics. (For more details...fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled neurons...
  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated by glutamate...they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation...
  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...clever system for reducing the on-target off-tumor activity of CAR–T cells was recently devised by the Yingxiao... plasmids here!   Wu Y, et. al. Control of the activity of CAR-T cells within tumours via focused ultrasound...
  4. How to Lead a Great Meeting

    Type
    Blog Post
    ...impacted by a project. Instead, invite the experts on the topic at hand. Invite one “ambassador” from each ...
  5. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ... including their potential toxicity and cross-reactivity with other pathways. Since these effectors are...chromosomally. Leakiness was also an issue; some promoter activity persisted in the inactive state. The dynamic range...
  6. Resume Writing for Non-academic Science Careers

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    Blog Post
    ... instead of having all the pages in a Curricula Vitae to showcase your publications, academic performances...perhaps you were involved in some extracurricular activities – did you volunteer to do science outreach and...
  7. A Deep Dive into BioBuilder

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    Blog Post
    ...How do I engage my students in real-world lab activities? I’m trying to inspire them and also keep up ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...
  8. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous publications...
  9. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post
    ...showing that Cpf1 can cleave an array of 4 crRNAs in vitro and when expressed in 293 cells. This allows for...effective method with multiple applications (in vitro, in vivo, transfection, viral transduction) for ...
  10. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient...
  11. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses researchers...mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes et al., 2011). The various ...
  12. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...the lacZ gene, thus disrupting 𝛽-galactosidase activity. These cells, presumably the cell you want, cannot...
Showing: 261 - 280 of 564 results