We narrowed to 455 results for: abo.5

...transgene. On a plasmid map, promoters are upstream or 5’ of the gene they control. Promoters can be organism...the Addgene blog Learn more about basic viral vector elements Read about four considerations for the ...Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best... Other cis-regulatory elements Next let’s talk about some of the elements that aren’t required parts ...the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression in retinal cells Resources...

...bioengineering 37.10 (1991): 989-993. PubMed PMID: 18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only downloadable... for the Addgene blog, I was focused on writing about new scientific techniques and cool plasmids. Creating... graphics were usually the last thing I thought about when writing posts. Since then I’ve realized my ...know of a tool or resource not mentioned? Tell us about it in the comments section!  Additional resources...scientists Find science career blog posts Learn about other lab software ...

... The sample was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity...Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...by flexibly deploying IgnaviCas9 without concern about its stability under harsh conditions. One example...

...Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...you choose the best CRISPR software for your needs 5 great apps for lab life  Sharing your lab protocols...particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular...other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer...

... patterns generated by the nickases; D10A creates 5’ overhangs and H840A creates 3’ overhangs in a PAM-out...Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips to Improve HDR Efficiency...

...proto-oncogene product. Molecular and Cellular Biology, 5(12), 3610–3616. doi: https://doi.org/10.1128/mcb.5.12.3610...Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed an improved voltage...

...increased my autonomy and satisfaction with my work.  5. Do you enjoy bench work? It’s easier to stay at the... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...overhangs depending upon whether they are on the 3’ or 5’ end of the gene of interest allowing for insertion...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...

...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment, hence... currently assumed that the cells came from an aborted fetus. HEKs have an unusual ploidy and are considered...

...single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed Central...measurement and it provides limited information about what materials or organelles are being targeted ...

... raising salaries as their website implies. These 5 organizations, according to the NSF data, account ...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...

...more important to start early. Think about your plan for the next 5 years. Does an academic career work...patent application processes? How about facilitating a collaboration between research groups? Perhaps ... a good job summarizing what a resume should be about. With a CV, information is usually presented chronologically...developed through your research? Don't simply write about the research you did. Emphasize the skills. If you... structures, then you might know a thing or two about computer modeling. Make sure to focus on these skills...the job you are applying for, in which case talk about your research, as an understanding of the topic ...you some advantage over other candidates. Write about your transferable skills Some of the skills you ...

...focusing on a task, like writing, and then take a 5-minute break. Each 30-minute interval is called a ...frequency of use in black. This paper talks more about the development of the De-jargonizer.   Academic...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast episode 2 ...Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation managers and...the pomodoro technique": This podcast talks more about using the Pomodoro method in grad school Additional...

...Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources on the Addgene blog Antibodies 101:...flexibility needed for multiplexing. In the case above, you can’t costain with your desired antibodies ...multiplexing with chimeric antibodies. In the case above you should use an anti-rat IgG1 secondary antibody...this blog post has answered all of your questions about the utility of isotype conversions and chimeric ..., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., &...

...emission maximum is 675 nm. mCarmine is monomeric, 4-5 fold brighter than mNeptune684, and has a favorable... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...emission maxima toward the red end of the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius...

...Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely soluble at RT. Track expression and purification...Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

... C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

...fluorescence, as the GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s. jGCaMP7f is the fastest...brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...