...relatively the same for quite a while and hadn’t kept pace with our growth over the last few years. Some of... professional, and modern. Instead of trying to place all of the content above the fold, we decluttered...elements and allowed our content to dictate the space. We wanted to comfortably tell our story, to get...

... a double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target... of the targeted region, whereas PAM-in designs place the PAMs closer together in the middle of the targeted...sites separated by 51-68 bp Use D10A for HDR Place intended insert in between nick sites Use 40 nt...

... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses...

...your workplace and you could end up making a huge impact on how waste is handled. A lot of places have...on waste, but also on culture. Encourage your workplace to become a leader in sustainability. Sign up...

... free online molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing... looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for...Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready...

...perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration of tangents...ideas and approaches. Moreover, the ability to embrace serendipity fosters adaptability, a crucial skill...your academic journey, remember to leave a little space for the unexpected — it might just be the key to...

...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...continue to help bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in...

... amazing job shuttling unwanted lab supplies to places that really need them.  When I was in graduate ...indefinitely at room temperature (thus also saving freezer space).  The procedure becomes less attractive when noting...very thin gel on a large glass slide using only surface tension.  It's a bit tricky to pour the right amount...

..."CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned...want to use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The...usually adds it when they order the oligo. If your spacer sequence starts with a 'G', you naturally have ...

... and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting Tenure...thing to do is not go into their labs in the first place. The good news There are many excellent mentors ...

... which studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization...the virus to gain entry into human cells via human ACE2 receptors. In their assay, the pseudoviruses express...reporter which is used as a readout for infection of ACE2-expressing target cells. Any neutralized pseudoviruses...

...tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ... graduate students with innovative ideas in all facets of the entrepreneurial process in order to develop...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your...

...about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a.m. many ...format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease case...they agreed on each element to use, they had to place the selected card on the board game. They had 35...

... that cell. Since there is a limited amount of surface area on each cell, the way to get more AAV in contact...expression. How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce ...concentration filter column, make sure to rinse off the surface of the filter membrane with the surrounding solution...

...where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it becomes... which is freely available online. Its simple interface can guide you through both experimental and primer...

...solution thawed, as the CO2 was confined to the tube headspace when the samples were frozen. This was clear by...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...tubes prior to thaw released the CO2 from the headspace, thus mitigating the pH effects of dry ice storage...

... limited by the need for a specific PAM (protospacer adjacent motif) in order to bind the target sites...mediated a C to T conversion in the target sites adjacent to an NG PAM in human cells. The increased versatility...

...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...

...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...complexity of antigen processing events that take place within an antigen-presenting cell like a T cell....

...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs developed...discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4...genes identified as non-essential. The knockouts replace entire protein-coding regions (excluding start ...