We narrowed to 344 results for: ada

1. All in a Twist: dsRNA

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   ...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...

2. Troubleshooting Your Plasmid Cloning Experiment

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   ...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but...

3. Plasmids 101: Cre-lox

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   ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

5. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

6. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

7. Adenoviral Vector Production and Troubleshooting

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   ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

8. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...triggers a cascade of events that results in the degradation of the target mRNA and silences its expression...

9. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

10. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

11. Hot Plasmids February 2024

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    ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

12. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    ...Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment...

13. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen...

14. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...Project One such space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace...

15. Hot Plasmids and Viral Preps - May 2021

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    ...validating this method through monitoring GFP degradation, this protein compartment was used to optimize...

16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...

17. Plasmids 101: Protein Expression

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    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

18. Truncated gRNAs for Regulating Gene Expression

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    ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

19. Plasmids 101: Knockout/Knock-In Plasmids

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    ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

20. Twenty Years of Addgene Sharing: CRISPR

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    ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...