Addgene

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Showing: 261 - 280 of 336 results

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Troubleshooting Your Plasmid Cloning Experiment

Type

Blog Post

...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but...
The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Type

Blog Post

...cells promote oxidative stress leading to the degradation of one key enzyme (among others): caveolin 1,...
Summer SciComm: Preprints

Type

Blog Post

...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...
All in a Twist: dsRNA

Type

Blog Post

...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...
Plasmids 101: Cre-lox

Type

Blog Post

...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

...triggers a cascade of events that results in the degradation of the target mRNA and silences its expression...
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Type

Blog Post

...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021. Let me leave...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Type

Blog Post

...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...
Hot Plasmids February 2024

Type

Blog Post

...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission. Find...
Science communication: 9 Strategies to Get Your Foot in the Door

Type

Blog Post

...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen...
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Type

Blog Post

...Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment...
Experimenting in an Open Source lab: from CRISPR to Cats

Type

Blog Post

...Project One such space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

Showing: 261 - 280 of 336 results

Results per page:

Troubleshooting Your Plasmid Cloning Experiment

The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Summer SciComm: Preprints

All in a Twist: dsRNA

Plasmids 101: Cre-lox

Adenoviral Vector Production and Troubleshooting

Educational Planarian Tools for Teaching Developmental Biology Techniques

Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Mouse Modeling, Part 1: Genetically Engineered Mice

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Hot Plasmids February 2024

Science communication: 9 Strategies to Get Your Foot in the Door

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Experimenting in an Open Source lab: from CRISPR to Cats

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: Knockout/Knock-In Plasmids

Plasmids 101: 5 factors to help you choose the right cloning method

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