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...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation...design for LIC is often as simple as using the backbone manufacturer's suggested leader sequence fused...

... pMAZ-SK contains an aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through...

... 37 °C for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded...

...Fluorescent Protein Plasmids by Category Empty Backbones Study your protein of interest by fusing it to...fluorescent proteins as well as hundreds of empty backbones. FRET Find FRET pairs and standards to study protein-protein...

...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi... are a set of plasmids all built with the same backbone and only differing in a small region. Click here...

...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...

...Browse shuttle plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production...Adenoviral Plasmids Browse commonly used adenoviral backbone plasmids for adenoviral vector production. ID ...

...most mammalian tissues; more hardy tissues such as bone, or bacteria/yeast/plant samples will require additional...

...reaction which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest...omitted from the coding sequence of the part and backbone. For some standards, there are additional sites...

...worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids... include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples...

...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597...

...the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com. When choosing...delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target ...

...protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human...RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library...

... W., & Gradinaru, V. (2023). Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable...

...All SGC Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Plasmids - Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...

...Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

..., and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

...Cloning protocols for using the pLKO.1 vector, a backbone used by the RNAi consortium for targeting human...