We narrowed to 310 results for: bon
-
TypeCollection...reaction which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest...omitted from the coding sequence of the part and backbone. For some standards, there are additional sites...
-
Worm Expression Resources
TypeCollection...worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids... include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human...RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library... -
CRISPR References and Information
TypeCollection...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597... -
Plan Your Experiment
TypeCollection...off-target editing is a major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the... to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and ... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post... W., & Gradinaru, V. (2023). Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable... -
CRISPR Pooled gRNA Libraries
TypeCollection...Library 169942 Knockout Human Ebert 3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR...CRISPR KO library 174592 Knockout Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection...this fusion protein guide to find the appropriate backbone). Then, by examining the overlap between your ... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol...Cloning protocols for using the pLKO.1 vector, a backbone used by the RNAi consortium for targeting human... -
Protocol - Bacterial Transformation
TypeProtocol...antibiotic resistance proteins encoded in the plasmid backbone so that they will be able to grow once plated ... -
Gibson Assembly Protocol
TypeProtocol...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009... -
Fluorescent Protein Guide: Biosensors
TypeCollection...Science. 2016 Jun 10;352(6291):1319-22. Tobias Bonhoeffer , Mark Huebener , Tobias Rose Calcium Soma-targeted...probe. Neuron. 2012 Sep 6;75(5):779-85. Vincent Pieribone Voltage ArcLight-ST to detect subthreshold membrane... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...where the strands start denaturing and the hydrogen bonds are broken between the nucleotide base pairs. The... -
Browse
TypeCollection...All SGC Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI... -
The SGC Oxford Collection
TypeCollection...Plasmids - Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI... -
The SGC Toronto Collection
TypeCollection...Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI... -
Cell Migration Consortium Plasmids
TypeCollection...Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI... -
TREAT-AD Plasmid Collection
TypeCollection..., and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI... -
Fluorescent Protein Guide: Activity Regulation
TypeCollection...Regulation More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics... -
MAPK Plasmids
TypeCollection...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...