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Showing: 261 - 280 of 310 results
  1. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...reaction which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest...omitted from the coding sequence of the part and backbone. For some standards, there are additional sites...
  2. Worm Expression Resources

    Type
    Collection
    ...worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids... include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples...
  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human...RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library...
  4. CRISPR References and Information

    Type
    Collection
    ...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597...
  5. Plan Your Experiment

    Type
    Collection
    ...off-target editing is a major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the... to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and ...
  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Library 169942 Knockout Human Ebert 3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR...CRISPR KO library 174592 Knockout Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries...
  7. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection...this fusion protein guide to find the appropriate backbone). Then, by examining the overlap between your ...
  8. Gibson Assembly Protocol

    Type
    Protocol
    ...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009...
  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Science. 2016 Jun 10;352(6291):1319-22. Tobias Bonhoeffer , Mark Huebener , Tobias Rose Calcium Soma-targeted...probe. Neuron. 2012 Sep 6;75(5):779-85. Vincent Pieribone Voltage ArcLight-ST to detect subthreshold membrane...
  10. Browse

    Type
    Collection
    ...All SGC Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...
  11. The SGC Oxford Collection

    Type
    Collection
    ...Plasmids - Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...
  12. The SGC Toronto Collection

    Type
    Collection
    ...Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...
  13. MAPK Plasmids

    Type
    Collection
    ...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...
Showing: 261 - 280 of 310 results