We narrowed to 333 results for: cki

1. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ...audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good questions with...

2. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...carried out much like the name implies — after nicking, Cas3 reels in the non-target strand of DNA much...

3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   ...prediction models. In addition, we provide options for nicking gRNAs (used for PE3 and PE5 prime editing strategies...

4. Of Myc and Men

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   ...involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds. But over the years...

5. Truncated gRNAs for Regulating Gene Expression

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   ...PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer modeling." Cell...

6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   ...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...

7. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...: 15582541. 5. Seale, P., A. Asakura, and M.A. Rudnicki, The potential of muscle stem cells. Dev Cell,...

8. 10 Basic tips for mammalian cell culture

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   ...wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather...

9. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus (see figure...

10. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...Who owns computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns ...

12. Plasmids 101: Secondary Nanobody Toolbox

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    ...available nanobodies from Pleiner et al. Before picking a secondary nanobody, it’s important to review ...

13. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...retrieved your lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now...

14. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...likely range from mildly surprising to genuinely shocking. Mainstream media outlets have addressed CRISPR...

15. DNA Purification Without a Kit

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    ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...

16. A Guide to Starting Your Own Journal Club

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    ...time to stay current or enjoy that discussion. Luckily, one needn't worry if they choose to move away ...

17. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have been used successfully (Kerppola, 2006...

18. Lentiviral Vector Uses and Overview

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    ...In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola D, Riecken K,...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ... molecules even enables us to skip the step of knocking-in. In 2013, we showed that isolation of a single...

20. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...