We narrowed to 334 results for: cki;
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TypeBlog Post...customers within 24 hours, and we’re pretty good about sticking to that. If you email us at noon and then call...
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Plasmids 101: FLEx Vectors
TypeBlog Post... 2003 PubMed PMID: 12665802. 2. Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of ... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...original plasmid which can over-inflate results. A packing vector deficient in reverse transcriptase will ... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...resulted in actionable tasks and I made a point of sticking to deadlines and performing the work I agreed ... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good questions with... -
10 Basic tips for mammalian cell culture
TypeBlog Post...wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...prediction models. In addition, we provide options for nicking gRNAs (used for PE3 and PE5 prime editing strategies... -
Of Myc and Men
TypeBlog Post...involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds. But over the years... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...carried out much like the name implies — after nicking, Cas3 reels in the non-target strand of DNA much... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...: 15582541. 5. Seale, P., A. Asakura, and M.A. Rudnicki, The potential of muscle stem cells. Dev Cell,... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer modeling." Cell... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus (see figure... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...Who owns computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...available nanobodies from Pleiner et al. Before picking a secondary nanobody, it’s important to review ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have been used successfully (Kerppola, 2006... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...likely range from mildly surprising to genuinely shocking. Mainstream media outlets have addressed CRISPR... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...retrieved your lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now... -
DNA Purification Without a Kit
TypeBlog Post... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...