We narrowed to 329 results for: cloning
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TypeBlog Post...protocols and guides regarding pooled libraries, from cloning a pooled library to viral production to screening...
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Lab to Office Culture Shock
TypeBlog Post... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start... -
Tips for arabidopsis transformation
TypeBlog Post...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium... -
AAVs in Retinal Gene Therapy
TypeBlog Post... Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 gene, a naturally occurring animal... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...experiment today! Get ready to identify knock-in clones by reading up on validating genome edits to find... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...control elements for each would be relatively large. Cloning the viral genes in this plasmid would be more difficult... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...yeast strain that also contains a reporter gene cloned downstream of a promoter containing the target ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...technique for molecular cloning, such as PCR - or restriction enzyme -based cloning. Protocol: Gel Purification... -
CRISPR Plasmids - Plants
TypeCollection... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ... -
TALEN Expression Vectors
TypeCollection...using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about... -
Video Library
TypeProtocol...technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do...Chain Reaction (PCR) You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post... gene for bacterial selection. The process for cloning the 20-nt guide RNA is identical for both vectors... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing ... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...SELEX). After 4-6 rounds of SELEX, the RNA pool was cloned into bacterial expression vectors to create a library... -
Zinc Finger Consortium: Nuclease Expression Vectors
TypeCollection... in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease... -
Antibody Guide
TypeCollection...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies... -
Plasmid Collections
TypeCollection... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...