We narrowed to 324 results for: cloning

... size, more durable, and usually much easier to clone, express, and purify compared to antibodies. Nanobodies...

...protocols and guides regarding pooled libraries, from cloning a pooled library to viral production to screening...

...experiment today! Get ready to identify knock-in clones by reading up on validating genome edits to find...

... If you don’t feel like doubling the amount of cloning, another option is to use variants of Cas9, like...

... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start...

...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...

... Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 gene, a naturally occurring animal...

...protein fusion. This one protein method simplifies cloning and makes your experimental setup less cumbersome...

...control elements for each would be relatively large. Cloning the viral genes in this plasmid would be more difficult...

...yeast strain that also contains a reporter gene cloned downstream of a promoter containing the target ...

...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

...technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA from an...

...our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do...Chain Reaction (PCR) You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation...

...virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing ...

... gene for bacterial selection. The process for cloning the 20-nt guide RNA is identical for both vectors...

...SELEX). After 4-6 rounds of SELEX, the RNA pool was cloned into bacterial expression vectors to create a library...

...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression. Select with puromycin or screen...

... Protocols Cloning by Limiting Dilution Isolating a Monoclonal Cell...transgene expression over time, as the lower expressing clones take over the polyclonal cell pool. Generating ...new window) or isolating individual cells with cloning cylinders. Workflow Timeline This protocol begins...

...vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the...

... or q, respectively). These sequences were then cloned into an AAV backbone containing a minimal promoter...