We narrowed to 376 results for: fus

...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...

... vector type  Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids...

...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...

...activated through administered drugs that must diffuse throughout the body, the onset of receptor activation...

... McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501...

...transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab...

...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over...

... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even...

...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy...

... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...

...Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources...

...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ...

...funded? Are there any financial aspects that are confusing? During a traditional campus visit, many students...

... proteins display a similar effect (many did!). Fusing EGFP to the self-labeling FlavinTag made a reliable...

... a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime...

...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...

...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability...

...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific...

... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these...

... dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information...peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a given...