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  1. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...confidential (see image below). Slide of information gathered by Postdoctoral Associations around Boston - From...who can provide additional advice to trainees navigating the waters of career development. Publishing ...individual is then judged objectively and possibly negatively by a third party, it can come as a shock to the...
  2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...photoinstability, as well as the tendency of fluorophores to aggregate in the neurons’ somata. Second, the system did...to find those with ideal characteristics (low aggregation, high photostability, and high stability after...immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine the number and type...
  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...extract the linearized band, ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric...version of the plasmid. Linearize, gel extract, and ligate the plasmid If you are having difficulty isolating...
  4. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...characterization, these proteins could function as surrogate antibodies for identifying and characterizing ...with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested vector designed for protein...presence of certain modifications to DNA bases.  Ligate and transform your resultant plasmid libraries ...
  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression...the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists used mutagenesis...function and localization, or leading to protein aggregation. Another important parameter to consider is the...
  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...page. The data produced is available on the F1000 gateway and Zenodo community.  Knockdown approach For... have the same clone available with different conjugates. Summary Flow cytometry is a powerful tool to...resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry...
  7. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish... diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors ...
  8. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...protocols.  These feature step-by-step instructions to navigate various lab processes.  Our most recent protocol...videos feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular...
  9. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...breakthrough research. Insights about how to navigate through all stages of your career, whether you...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...
  10. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system, Georgieva...overexpression, while the rest of the fusions had some negative effect on these parameters. IT5 and IT6 displayed...
  11. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...developed and implemented by applying the Golden Gate cloning method to human proteins known as Pumilio...lose their solubility. Her lab is currently investigating the issue and hopes to have a better understanding...
  12. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...weekend in the United States, I look forward to gathering around a large table for a meal with my family...thankful for many things, and one is that I get to gather around a large table to eat with my Addgene family...
Showing: 261 - 280 of 625 results