...confidential (see image below). Slide of information gathered by Postdoctoral Associations around Boston - From...who can provide additional advice to trainees navigating the waters of career development. Publishing ...individual is then judged objectively and possibly negatively by a third party, it can come as a shock to the...

...photoinstability, as well as the tendency of fluorophores to aggregate in the neurons’ somata. Second, the system did...to find those with ideal characteristics (low aggregation, high photostability, and high stability after...immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine the number and type...

...extract the linearized band, ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric...version of the plasmid. Linearize, gel extract, and ligate the plasmid If you are having difficulty isolating...

...characterization, these proteins could function as surrogate antibodies for identifying and characterizing ...with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested vector designed for protein...presence of certain modifications to DNA bases.  Ligate and transform your resultant plasmid libraries ...

...yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression...the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists used mutagenesis...function and localization, or leading to protein aggregation. Another important parameter to consider is the...

...page. The data produced is available on the F1000 gateway and Zenodo community.  Knockdown approach For... have the same clone available with different conjugates. Summary Flow cytometry is a powerful tool to...resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry...

...covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish... diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors ...

...the blog’s newly updated navigation menu and search tool to help you navigate the scavenger hunt. Good...

... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...protocols.  These feature step-by-step instructions to navigate various lab processes.  Our most recent protocol...videos feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular...

...breakthrough research. Insights about how to navigate through all stages of your career, whether you...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...

...electricity aggregation program” for all local households and businesses. Electricity aggregation allows a...

...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...collaborative nature of working in industry and how delegating can empower people Chapter 3: How Crystal advanced...

.... Looking for something? Use the navigation menu. The new navigation menu showcases the five main topics...

...in human cells They used the Golden Gate cloning method to ligate expression cassettes of the gRNAs into...

...expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system, Georgieva...overexpression, while the rest of the fusions had some negative effect on these parameters. IT5 and IT6 displayed...

...developed and implemented by applying the Golden Gate cloning method to human proteins known as Pumilio...lose their solubility. Her lab is currently investigating the issue and hopes to have a better understanding...

...Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro... Our most popular kit of the year was the Golden Gate TALEN Kit, which continues to be our most requested...

...weekend in the United States, I look forward to gathering around a large table for a meal with my family...thankful for many things, and one is that I get to gather around a large table to eat with my Addgene family...

... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...