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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 297 results for: hal.2

Showing: 261 - 280 of 297 results

  1. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...annurev-neuro-111020-100834 Challis, R.C., Ravindra Kumar, S., Chan, K.Y., Challis, C., Beadle, K., Jang, ...or lumbar spine. Image and caption courtesy of Challis et al., 2022. Where do they come from? Systemic...https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com. ...low doses a regional bias towards the cortex and thalamus emerges. AAV-PHP.eC effectively transduces neurons...

  2. RNA Extraction Without A Kit

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    Blog Post
    ...156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

  3. Antibodies 101: Affinity Reagents

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    Blog Post
    ...Antibody Fragments. Antibodies (Basel). 2019 Apr 9;8(2):28. doi: 10.3390/antib8020028. PMID: 31544834; PMCID...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’re more ...

  4. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...RNA is not inherently fluorescent and can be challenging to track intracellularly, but aptamers permit... when bound to DFHBI despite being approximately half the length (Warner et al., 2014); its length reduces...next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing...

  5. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...this year. Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post
    ...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

  7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...excise the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al...be the way to accomplish this tough job! Kamel Khalili’s lab at Temple University has demonstrated two ... a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence specificity..., Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation...T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid...PMID: 26035283. PubMed Central PMCID: PMC4649911. Khalili K, Kaminski R, Gordon J, Cosentino L, Hu W. Genome...

  8. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...15.4%) 46 (59%) of these students were in the last 2 years of their degree. Students filled in a pre-program...PhD, achieving our career goals can be a real challenge. On top of that, in the midst of a rapidly changing...mentees with a broader, first-hand vision of the challenges and career opportunities that truly exist outside...was much more than that. I discovered that the challenges that I am facing in my career are common problems... that many others have faced yet, still, this challenge did not hinder them from advancing their careers...

  11. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    .... Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the...

  12. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) General protein expression...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131...

  13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...improvement in muscle physiological performance.  2. Trans-splicing Overview: For trans-plicing, splice...packaging capacity is limited to ~4.7kb, or roughly half the packaging limits of lentiviral and adenoviral...multiple copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440...heterodimer, then splicing of the pre-mRNA of the two halves of the transgene will reconstitute the full-length...A of the transgene contains the promoter, the 5’ half of the transgene, a splice donor sequence and the... of homology, a splice acceptor sequence, the 3’ half of the transgene. The full length gene is reconstituted...124–130 . https://doi.org/10.1038/sj.mt.6300322 Halbert CL, Allen JM, Miller AD (2002) Efficient mouse ...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...delivering CRISPR components as RNPs and delivering two halves of a repair template spread across two AAVs, it...

  15. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post
    ...You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

  16. Of Myc and Men

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    Blog Post
    ...promising monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your antibody...in these species, they found that the N-terminal half of the antigen differs significantly between human...

  18. Transferable Skills Guide: Managing a Team

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    Blog Post
    ...most important” tasks my schedule. This was bad for 2 reasons. First, it soon became obvious that I didn.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...

  20. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post
    ..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...properties of the protein of interest can be challenging. In this blog I discuss strategies to generate... (catalytic activity or interactions) can be challenging. If a fusion can complement a knock-down or knock-out...
Showing: 261 - 280 of 297 results