Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 560 results for: mme

Showing: 261 - 280 of 560 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...a single gene they want to activate, we would recommend using a cDNA overexpression vector rather than...closest to the transcription start site (TSS). We recommend targeting the guides to a region less than 200...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... mycoplasma contamination. There are several commercially available mycoplasma detection kits that use... example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced ...

  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...countries around the world and to do so, the WHO recommends one strategy: testing, tracking, and social distancing...recombinase polymerase amplification (RPA) using a commercially available amplification kit. In this case, regions...

  5. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...antibodies and refer to the manufacturer’s recommendations.  As you optimize your protocol, always remember...To learn more about antibody validation, this commentary by Uhlen, et al. 2016 is a good place to start...

  6. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email.... These should be plainly stated. I would not recommend putting these into slide format. You want people...

  7. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...something to brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots...about your favorite new biosensor plasmids in the comments below!   Additional resources on the Addgene ...

  8. Evolution of Lab Techniques

    Type
    Blog Post
    ...into the field. Current versions are already commercially available although they are reported to have...your favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger...

  9. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... before you start an experiment with CRISPR The hammer, the jigsaw, and the wrench are all great tools...ignored. Created with BioRender.com. The hammer: Gene knockout by NHEJ Gene knockout with CRISPR...

  10. A Control for All Seasons

    Type
    Blog Post
    ...just natural differences between individuals. Recommended controls for antibody-based experiments Primary...above, viability and compensation controls are recommended for flow cytometry. A viability control allows...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    ...predict the perfect titer, but one of the main recommendations when using AAVs is to avoid freezing and thawing... ScAAV avoid this rate limiting step and can immediately undergo replication and transcription. However...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... Additional libraries are also available from commercial vendors. These libraries usually contain 4-12...community. It shows how science can be accelerated immensely when we share knowledge and reagents. I have not...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...distinguish a signal from noise; therefore, it is recommended to use brighter fluorophores for markers that...marker to be highly abundant, it is fine to use a dimmer fluorophore. However, these aren't strict rules...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...residues. While these Cy/NTA conjugates are not commercially available, they are easily synthesized with ...Griffin, Adams, & Tsien, 1998), these dyes are now commercially available as Lumio Green and Lumio Red from ...

  16. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... are accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic...optimal time period between viral injection and commencement of behavioral experimentation.  Whether you ...

  17. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics of...

  20. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...the seventh day of CRISPR, Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease...
Showing: 261 - 280 of 560 results