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We narrowed to 575 results for: mme

Showing: 261 - 280 of 575 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ...Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured...

  6. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...librarians and data curators, IT managers, the commercialization office, the CIO’s office, and research computing...of the vibrant life sciences research and commercialization community in greater Boston, while at the ...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...bevacizumab has recently been added to the treatment recommendations for non-resectable mesothelioma by the National.... Senate. If this bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that...

  11. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...we hear the word ‘antibiotic’, very often we immediately imagine a prescription for a bacterial infection...drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & HM Government (2016...

  12. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

  13. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...a single gene they want to activate, we would recommend using a cDNA overexpression vector rather than...closest to the transcription start site (TSS). We recommend targeting the guides to a region less than 200...

  14. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... mycoplasma contamination. There are several commercially available mycoplasma detection kits that use... example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced ...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...something to brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots...about your favorite new biosensor plasmids in the comments below!   Additional resources on the Addgene ...

  16. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...antibodies and refer to the manufacturer’s recommendations.  As you optimize your protocol, always remember...To learn more about antibody validation, this commentary by Uhlen, et al. 2016 is a good place to start...

  17. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email.... These should be plainly stated. I would not recommend putting these into slide format. You want people...

  19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...countries around the world and to do so, the WHO recommends one strategy: testing, tracking, and social distancing...recombinase polymerase amplification (RPA) using a commercially available amplification kit. In this case, regions...

  20. Evolution of Lab Techniques

    Type
    Blog Post
    ...into the field. Current versions are already commercially available although they are reported to have...your favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger...
Showing: 261 - 280 of 575 results