...selecting a lab with a PI who is a good mentor is important in both cases. As a PhD student you need to learn...scientists. The specific research area is not as important as choosing a supportive, positive role model....future career, so the research topic will be more important. In addition, it is crucial that you choose an...

...potentially be used to report upon and/or engineer “temporally ordered biochemical events” such as those that...study basic virus biology while leaving virulent components behind. Minigenomes are stripped down viral genomes...School describes how AAVs function and indicates important things to consider if you’re getting started with...

...year. Introducing Arabidopsis Arabidopsis is an important model system for research and is used by scientists... to say that most people do not recognize its importance or perhaps even know it exists. Couple that with...science festivals, ABRC gets the word out about the importance of plant sciences. In 2017 alone, ABRC staff ...

...designing your experiment, read our blog post on important considerations when using AAVs. Experimental results... we also take negative data, which is equally important to report. We recently published a paper in Frontiers... hub! Related resources on the Addgene blog Important Considerations When Using AAVs Three Key Considerations...

...reaction Run PCR to amplify your insert DNA. It is important to use a high fidelity polymerase to minimize .... The fidelity of the polymerase becomes more important the longer the expected PCR product is. You should...used. No matter which polymerase you use, it is important that you sequence the final product. Additional...

... interviews Studying for interviews is really important. For my Postdoc position, I tried to read at least... current sales position interview was just as important. The problem was I knew I had a knowledge gap....teaches you about selling products which is an important part of the business and allows you to work with...

...said. “We know CREB is really important, but where is it really important and when? That is not known.”...interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected, and they...

...color switching, TRICK requires the following components: a GFP protein that binds to the coding region... reporter. Delayed translation is known to be important in development, and Halstead et al. used TRICK...and 3’ UTR. Before beginning experiments, it’s important to verify that these changes do not impact translation...

...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

...attention for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, ..., From Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports...., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci., 113, 9882-9887...

...it can be important to consider the type of bacteria it is being used in, and how components of the plasmid... bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria, ...

... for favors.  Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...because I want it to be clear that it is completely impossible for me or anyone to stay in touch with all of...

... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

...proteins consuming molecular fuel sources. While the importance of understanding these complex processes can’...degradation under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were...

...that no component of the overall project gets left undone or is duplicated. With each component of the ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ...

...This is followed by some perhaps surprising, yet important, factors to consider when seeking your dream lab...short-term goals and chat about awesome science. Important factors in the search for a lab Studies show that...postdoc. Although the chosen research field is important, the end goal during this time is to be trained...

...only examined under a few conditions since it is impossible to check all possible combinations of biology...detected signal and therefore high brightness is an important feature of a fluorophore. The key question is ...However, the theoretical brightness ignores several important experimental conditions that are related to the...

... fluorescence, so higher concentrations of the compound can be added to the media. Cellular esterase then...producing a constant supply of substrate. Using this compound to excite the Nano-lanterns, Takai et al. continuously... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...

...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications. Challenges...

...most important resource that Addgene provides the scientific community? Katz: The most important resource...beyond. In your perspective, what do you think are important considerations for the future of open science?...