We narrowed to 760 results for: mpo
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TypeBlog Post...selecting a lab with a PI who is a good mentor is important in both cases. As a PhD student you need to learn...scientists. The specific research area is not as important as choosing a supportive, positive role model....future career, so the research topic will be more important. In addition, it is crucial that you choose an...
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Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...year. Introducing Arabidopsis Arabidopsis is an important model system for research and is used by scientists... to say that most people do not recognize its importance or perhaps even know it exists. Couple that with...science festivals, ABRC gets the word out about the importance of plant sciences. In 2017 alone, ABRC staff ... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...potentially be used to report upon and/or engineer “temporally ordered biochemical events” such as those that...study basic virus biology while leaving virulent components behind. Minigenomes are stripped down viral genomes...School describes how AAVs function and indicates important things to consider if you’re getting started with... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...designing your experiment, read our blog post on important considerations when using AAVs. Experimental results... we also take negative data, which is equally important to report. We recently published a paper in Frontiers... hub! Related resources on the Addgene blog Important Considerations When Using AAVs Three Key Considerations... -
Plasmid Cloning by PCR
TypeBlog Post...reaction Run PCR to amplify your insert DNA. It is important to use a high fidelity polymerase to minimize .... The fidelity of the polymerase becomes more important the longer the expected PCR product is. You should...used. No matter which polymerase you use, it is important that you sequence the final product. Additional... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post... interviews Studying for interviews is really important. For my Postdoc position, I tried to read at least... current sales position interview was just as important. The problem was I knew I had a knowledge gap....teaches you about selling products which is an important part of the business and allows you to work with... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...said. “We know CREB is really important, but where is it really important and when? That is not known.”...interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected, and they... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...it can be important to consider the type of bacteria it is being used in, and how components of the plasmid... bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria, ... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post... for favors. Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...because I want it to be clear that it is completely impossible for me or anyone to stay in touch with all of... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment. ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address). One may also simply attach important references that serve to further characterize ... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...color switching, TRICK requires the following components: a GFP protein that binds to the coding region... reporter. Delayed translation is known to be important in development, and Halstead et al. used TRICK...and 3’ UTR. Before beginning experiments, it’s important to verify that these changes do not impact translation... -
K. phaffii: Rising to the Occasion
TypeBlog Post...attention for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, ..., From Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports...., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci., 113, 9882-9887... -
5 Great Apps for Lab Life
TypeBlog Post... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...proteins consuming molecular fuel sources. While the importance of understanding these complex processes can’...degradation under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were... -
Management for Scientists: Delegating is Key
TypeBlog Post...that no component of the overall project gets left undone or is duplicated. With each component of the ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ... -
Finding and Joining Your Dream Lab
TypeBlog Post...This is followed by some perhaps surprising, yet important, factors to consider when seeking your dream lab...short-term goals and chat about awesome science. Important factors in the search for a lab Studies show that...postdoc. Although the chosen research field is important, the end goal during this time is to be trained... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...library used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that...variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications. Challenges... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...most important resource that Addgene provides the scientific community? Katz: The most important resource...beyond. In your perspective, what do you think are important considerations for the future of open science?... -
Plasmids 101: Screens vs. Selections
TypeBlog Post...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...tools are not very robust. When using GCE, each component of the experiment system (including media, cell...optimization parameters the same for all three components, meaning that the final protocol may involve ... They provide resources, knowledge, and most importantly, validated protocols (using readily available...