We narrowed to 290 results for: nif

...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...

...owning. Conflict in this latter scenario usually manifests as the assumption “I thought that was X’s job”...

...clones, it’s wise to quickly assess whether a significant number of cells have been edited. For many experiments...

...double-stranded breaks in the DNA backbone, making them significantly less prone to indel mutations that plagued CRISPR...

...On this gel, the protein of interest band is significantly lighter in “condition 1” than “condition 2.”...

...kidney development. Despite their ubiquity and significance, these receptors have proved challenging to ...

...figure). Template A high purity plasmid prep significantly increases the success rate of site directed ...

...-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, although the ...

...they all start with a G!) but actually work by significantly different methods. Golden Gate cloning does ...

...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...

...few replicates leading to suspect statistical significance (you can read more about this problem in Button...

...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...

...and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, and sensitivity...

...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

...However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA...

...the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you...

...should appear less green. Note that there won’t be uniform editing across all the cells in the population,...

...depending on how and where you pursue it. It can manifest via research and data gathering, regulatory efforts...