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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 658 results for: otos

Showing: 261 - 280 of 658 results

  1. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against your target of...… Another strategy for species-on-species IHC protocols is to block endogenous IgGs by pre-treating the...

  2. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...General Use Antibodies Antibody guide Antibody protocols Additional resources on the Addgene blog Antibodies...

  5. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

  7. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable The lab of Michael Davidson from Florida State...new window) for images, tutorials, microscopy protocols, and more. Empty Backbones Plasmid Description...Plasmid Description Industry Return to top Photoactivatable Plasmid Description Industry Return to top...

  10. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  11. Components of CRISPR/Cas9

    Type
    Blog Post
    ...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector...

  15. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

  16. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

  17. Antibodies 101: ChIP

    Type
    Blog Post
    ...from the difficulty of developing an optimized protocol - it’s a bit like Goldilocks and the Three Steps...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References...

  19. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... indels that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases ...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

  20. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...well-suited for use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this...TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically modified...
Showing: 261 - 280 of 658 results