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Showing: 261 - 280 of 660 results

  3. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

  5. Plan Your Experiment

    Type
    Collection
    ...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...10.1038/s41587-020-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/...

  6. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  9. Components of CRISPR/Cas9

    Type
    Blog Post
    ...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...

  11. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

  12. Antibodies 101: ChIP

    Type
    Blog Post
    ...from the difficulty of developing an optimized protocol - it’s a bit like Goldilocks and the Three Steps...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector...

  17. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... indels that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases ...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

  18. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...well-suited for use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this...TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically modified...

  19. TALEN Plasmids and Kits

    Type
    Collection
    ... 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory...Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN... at the final assembly step in the Golden Gate protocol. Both plasmids 47388 and 47389 include a Flag ...

  20. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. The next time you...academic labs when creating shared experimental protocols. Clarify: Asking for clarification during a meeting...
Showing: 261 - 280 of 660 results