...posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors in your...of your choice. More information and a detailed protocol on how to use the pLKO.1 vector can be found here...

.... Recent studies have shown that the conditions Sotos-type 2 and Marshall Smith Syndrome are due to mutations...more accurate diagnosis of NFIX-related syndromes (Sotos-type 2 and Marshall-Smith) and could be expanded...

...fluorescence intensity due to interactions with photoactivatable fluorescent protein Dronpa. The authors tracked...peak of 519 nm. These proteins have improved photophysical properties and can be used in quenching, FRET...

...a group discussion on the best strategies and protocols for different experiments.  In this blog post...and resources page Browse our molecular biology protocols Read our molecular biology reference ...

...to ABE7.10. Depending on the position in the protospacer, the editing capacity of the ABE8s ranged from...site in the genomic DNA to contain a specific protospacer adjacent motif (PAM) made up of 5′-NGG-3′ (“N...

...contact information, and potentially a photograph of yourself. A photo can be very useful in the event that...

...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI:10.1038/nprot.2016.171 ...

...Check out our AAV Production Protocol Check out our AAV Purification Protocol ...

...encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, ...genetically targetable fluorescent indicators. Nature protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org...

...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...with the IPD of a cation channel, such as the serotonin 3 receptor, will promote depolarization in neurons...

...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody...sample. There are species-on-species staining protocols that use antibody fragments to block the endogenous...

...reported the development of a rapid design and prototyping pipeline that can take pathogen sequence information...critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future emerging...

... Lab TAP Tagging Protocol 97.2 KB Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited...provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB Mendenhall...can be found in Sakuma et al., Nature Protocols, 2016 . Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...tagging techniques, as well as the plasmids and protocols needed for using them, are described below. Read...2014 The Förstemann lab has provided detailed protocols for N- or C-terminal tagging in Drosophila cells...locus in C. elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no ...

...Titration of baculovirus can vary depending on the protocol used. Plaque assays are standard but can take ...More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources References Shaw, ...

..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto, T. (2023) Single-Molecule Magnetoluminescence...

...taking a snapshot of chromatin looping with the photo “developed” by analyzing DNA sequencing data. While... The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0 allow us to uncover...

...error. Used in combination with standard barrier protocols, they can reduce the risk of accidental escape...research have conclusively shown that barrier protocols are vulnerable to human error. And with gene drives...

...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

... multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and are...690/717 (after photoactivation) 3.2 pPAiRFP1-N1 PAiRFP2 692/719 (after photoactivation) 3.0 pPAiRFP2-N1... imaging using far red, near-infrared, and photoactivatable fluorescent proteins. Plasmid...imaging are derived from bacterial phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent...The following far red , near-infrared , and photoactivatable fluorescent empty backbone plasmids are ideal...piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find...

...Gamma-protocadherin-A3 [N144/5R] Gamma-protocadherin-A3 Mouse Mouse IgG2a 222155 Gamma-protocadherin-B2 ...Mouse IgG2a 164156 Anti-Gamma-protocadherin-C3 [N174B/27R] Gamma-protocadherin-C3 Mouse Mouse IgG2a 164157... 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504...IgG2a 182105 Anti-Pan-Gamma-protocadherin-A [N144/32R] Pan-Gamma-protocadherin-A Mouse IgG2a 182106 Anti-Histone...Mouse IgG2a 190299 Anti-Gamma-protocadherin-B2 [N148/30] Gamma-protocadherin-B2 Mouse Mouse IgG2a 190300...Mouse IgG2a 199398 Anti-Gamma-protocadherin-A3 [N144/17R] Gamma-protocadherin-A3 Rat Mouse IgG2a 199399 Anti-PINK1...Pan-Gamma-protocadherin-Constant recombinant mouse monoclonal antibody. Pan-Gamma-protocadherin-Constant...