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... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby.   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby. The ease of observing...

...endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against your target of...… Another strategy for species-on-species IHC protocols is to block endogenous IgGs by pre-treating the...

... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...General Use Antibodies Antibody guide Antibody protocols Additional resources on the Addgene blog Antibodies...

...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

...Database, bas006 (2012). PubMed. For a stepwise protocol on designing and using RNA scaffolds: Delebecque...scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

... the workshop, the participants took part in a protocol drawing exercise. The workshop also used a shared...different parts of the poster. Conferences, such as Photonics Online Meetup, hosted their poster session on ...

...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...

...verification to find additional tips and detailed protocols on topics such as how to set up your digests and...Addgene's Molecular Biology Reference Find More Protocols and Videos      ...

...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

...from the difficulty of developing an optimized protocol - it’s a bit like Goldilocks and the Three Steps...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References...

...2017): 113-117. PubMed PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma...

...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector...

... indels that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases ...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

...Besides being compatible with any vector, the SLIC protocol itself is flexible. For one thing, those 5′ overhangs...Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular...

...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

...well-suited for use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this...TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically modified...

... with most multi-labeling immunohistochemical protocols where either a directly labeled primary or a primary...secondary antibody. Compatible with multi-labeling protocols when other antigens are detected with either a...