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Showing: 261 - 280 of 783 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin... This post was updated on Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected...Bacterial Expression Systems. Check out our companion post describing protein expression strains, where we ...

  2. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...viral service page and browse our viral vector blog posts for many more tips and ideas on how to use these...Expression in Mammals Subscribe to Viral Vector Blog Posts Additional Resources on Addgene.org Check out ...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...protocols for different experiments.  In this blog post let's take a look at some of the other open resources...   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study...Cas9 in C. elegans Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids...

  4. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ... sure you’re familiar with the policies before posting something on social media. If someone is presenting...and Meet People at a Scientific Conference” blog post. There’s so many great tips for getting the most...conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific communication,...you have a poster or talk at the conference, you can end by asking them to come to your poster or talk. ...be beneficial. The poster session Before the conference If you’re presenting a poster at a conference, ...people will visit your poster: those that want you to walk them through the entire poster, or those that want... the poster) so your visitors can have a DOI to go to. During the conference Arrive at the poster session...

  5. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit spreadsheet...“How to Deposit Your Plasmids with Addgene” blog post for a thorough guide to our online data entry process...discoveries with scientists around the world. This post was originally written by Tyler Ford in March 2017...

  6. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... find themselves faced with a roadblock. In this post, we will provide an overview of some of the common... tips on lentiviral titering please see our blog post. Between choosing the best lentiviral system, producing... to check out the lentiviral protocols and blog posts on Addgene’s website for additional resources. ...

  7. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...initiation, elongation, and termination. In a previous post we discussed the promoter's role in the initiation...ultimately gene-expression. Polyadenylation is the post-transcriptional additional of multiple adenine (...Cloning Techniques Read all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference...Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of...

  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...This post was contributed by guest blogger Derek Simon. The surgeries and standard molecular neuroscience...excitation spectrum (use the resources discussed in post one of this series already provided or check the... to the fun stuff: the experiments! In our final post in this primer on optogenetics we’ll delve into ...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  9. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...This post was contributed by Dora Farkas of www.FinishYourThesis.com and is one of many posts in our ...career articles on our blog Read Joanne Kamen's Post on What Makes a Good Mentor Resources on the Addgene...the needs of other people. Some students and postdoctoral fellows are hesitant about expressing their ...

  10. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...Biophysics from UC Davis in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University...Terriers. More SynBio Reading Read more Addgene blog posts about synthetic biology ...was facing the prospect of having to do a third postdoc in order to rebuild my resume, so I had started...

  12. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ... a team. As described here in a 2012 Intuit blog post, a study coming out of MITs Human Dynamics Laboratory...effective delegation will be covered in a future post in this series). Members carry on back-channel...

  13. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding your most used protocols & references...wild with sticky notes and labels. Color code your Post-its and write brief descriptions on each. Place ...

  14. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...significant pairings, additional tests, referred to as post-hoc analysis, must be performed. These will be discussed... choosing an ANOVA/MANOVA statistical test   Post hoc tests Knowing that significance exists somewhere... multiple groups is rarely enough information. A post hoc test, done after your initial analysis can provide...ANOVAs/ANCOVASs must be performed first, and then post hoc tests can be run on these tests. All sample...

  15. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...This post was contributed by guest blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics ...researcher in the Lu Lab at MIT. Note: The following blog post reduces the content of our paper, “Synthetic recombinase-based...they can control recombinase activity. For example, post-transcriptional recombinase activation via RNA toehold...Student to SynBio Entrepeneur  Read All of Our SynBio Posts Additional Resources on Addgene.org Visit Our ...

  16. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning Qin and... this process check out our Mouse Modeling blog posts: Part 1: Genetically Engineered Mice and Part 2:...50% when using ESC (Cohen, 2016). See Our Recent Post for Tips on CRISPR Gene Editing in Mice Disadvantages...additional advice from John Doench’s gRNA design blog post. Finally, always remember that you are working with...

  18. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ... of another academic year, graduate students and post-docs alike are wondering where the time has gone....2015.04.025. PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong Science...planning help alleviate the anxiety of graduate and postdoc training. On the other side, the PI has an opportunity...

  19. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...cleavage sites should one need to remove the tag post-purification. A sample of pCri constructs: pCri...to glycosylate proteins and may recapitulate the post-translational modifications added to proteins in...
Showing: 261 - 280 of 783 results