We narrowed to 334 results for: uros

1. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   ... https://doi.org/10.1038/nature14299  Senís E, Fatouros C, Große S, Wiedtke E, Niopek D, Mueller A-K, ...

2. Hot Plasmids - August 2020

   Type

   Blog Post

   ...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...

3. Antibodies 101: Reading a Flow Plot

   Type

   Blog Post

   ...Moya-Quiles, M., Minguela Puras, A., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and...

4. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   ...reporter gene examples are below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful...

5. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   ...the genetic basis of evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) ...

6. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   ...expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_...

7. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   ...pluripotent stem cells (Chavez et al., 2015) into neuronal cells by activating genes that cause differentiation...

8. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   ...Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A. viscosus, Aerobacter...

9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

   Type

   Collection

   ... plasmids with Neomycin selection Puromycin Mammalian Tet-pLKO-puro - Tet-inducible lentiviral...Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning...plasmids for Drosophila expression pMT-puro - Inducible gene expression under...mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1...lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA...lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral...

10. Zhang Lab CRISPR Page

    Type

    Collection

    ...targeting efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n...2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A...) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR ...PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; ...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958...-mediated expression of an sgRNA targeting the neuronal-specific gene NeuN. As a control they designed...

11. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016...

12. Hot Plasmids: Summer 2024

    Type

    Blog Post

    ...for labeling these cells. Check out Addgene’s Neuroscience Antibody Collection to find a recombinant anti-GFAP...

13. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    ...have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016). For these...

14. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ...Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503...

15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    ...with an in vitro transposition reaction." BMC neuroscience 3.1 (2002): 7. PubMed PMID: 12086589. PubMed...

16. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    ...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...

17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    ...potential tools for eradicating HIV-1/AIDS. J Neurovirol. 2015 Jun;21(3):310-21. PubMed PMID: 25716921...

18. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ...23663447. PubMed Central PMCID: PMC3684516. 5. Casini, Arturo, et al. "One-pot DNA construction for synthetic...

19. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    ...antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. 2014 Feb;24(1):...

20. Luciferase Plasmid Collection

    Type

    Collection

    .... Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling...firefly luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly...Venus-Aka-luciferase Roland Friedel 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of Renilla luciferase...luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of Venus-firefly...for bioluminescent chemo-optogenetic control of neuronal signaling. TRUPATH Kit , ONE-GO Biosensors Kit...