We narrowed to 333 results for: uros
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TypeBlog Post...reporter gene examples are below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful...
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What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...the genetic basis of evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...gutter or the bottom of Tokyo Bay. When a weird, sulfurous smelling, black pellet was sent to the AbuGoot... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A. viscosus, Aerobacter... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...Moya-Quiles, M., Minguela Puras, A., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and... -
Plasmids 101: Gateway Cloning
TypeBlog Post...expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_... -
CRISPR Activation: A Practical Guide
TypeBlog Post...pluripotent stem cells (Chavez et al., 2015) into neuronal cells by activating genes that cause differentiation... -
AAV Molecular Tools
TypeCollection...Eric Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology experiments...Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV...expression of Kir2.1 and mScarlet-KASH for silencing neurons 8 Ivo Spiegel Anterograde Transsynaptic Tracers...direction across synapses. Also see Monosynaptic Neuronal Tracers available at Addgene. ID Name Expression...protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016... -
Five Popular Model Organisms, Part 2
TypeBlog Post...have also been routinely used in an array of neuroscience research (Ellenbroek and Youn, 2016). For these... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503... -
Hot Plasmids: Summer 2024
TypeBlog Post...for labeling these cells. Check out Addgene’s Neuroscience Antibody Collection to find a recombinant anti-GFAP... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...with an in vitro transposition reaction." BMC neuroscience 3.1 (2002): 7. PubMed PMID: 12086589. PubMed... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...potential tools for eradicating HIV-1/AIDS. J Neurovirol. 2015 Jun;21(3):310-21. PubMed PMID: 25716921... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...23663447. PubMed Central PMCID: PMC3684516. 5. Casini, Arturo, et al. "One-pot DNA construction for synthetic... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. 2014 Feb;24(1):... -
Retrovirus Plasmids
TypeCollection...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression. Select with puromycin or screen...creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid 1774 for neomycin selection...expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A...47916 pRXTN CMV/MSV A modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart 20672...GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid. Deletion... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro - ...Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning...plasmids for Drosophila expression pMT-puro - Inducible gene expression under...mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1...lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA...lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral...