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Showing: 261 - 280 of 337 results

  3. Brain Armamentarium

    Type
    Collection
    ...protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan Ting...collection of Armamentarium tools will aid neuroscientists in studying circuits in laboratory animals ...technologies have the potential to transform neuroscience research by bringing the precision of molecular...Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862-PHPeB AiP14825...Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB AiP14496...separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857-PHPeB AiP14361...jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru 214856...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459...pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Zhang ...pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795 Mammalian/Lentiviral U6 none S. pyogenes Puro Zhang...Lentiviral BsmBI yes, cut S. pyogenes Puro Zhang pKLV-U6gRNA(BbsI)-PGKpuro2ABFP 50946 Mammalian/Lentiviral BbsI...Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-...pyogenes Puro Ebert pL-CRISPR.EFS.PAC 57828 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC...pLenti SpBsmBI sgRNA Puro 62207 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Maehr pKLV-U6gRNA-EF(...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...functionally distinct interneurons in mice, non-human primates and humans. Nat Neurosci. 2020 Dec;23(12):1629...targeting and manipulating interneurons across vertebrate species. Nat Neurosci. 2016 Oct 31. Gordon Fishell...Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine...for glutamatergic neurotransmission Targeted sensors for glutamatergic neurotransmission. eLife. 2023 Jan...mouse hippocampal neurons. eLife. 2020 Jun 9;9. pii: 56469. Edwin Chapman Neuropeptides GRAB biosensors ... orexin neuropeptides A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nat Methods...expression in neurons A genetically encoded sensor for measuring serotonin dynamics. Nat Neurosci. 2021 Apr...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...Fibroblasts Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons by defined ...Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons. Proc Natl...Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons. Nature....Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert...cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun...Fibroblasts Medium Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent...

  9. Antibodies 101: Validation

    Type
    Blog Post
    ...Torensma R, Turley H, Pulford K, Banham AH (2015) The European antibody network’s practical guide to finding ...

  11. Deisseroth INTRSECT Collection

    Type
    Collection
    ...2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci. 23(1):61-74. PubMed (Link opens...defined dopamine neuron subtypes using intersectional genetic approaches. Nat Neurosci. 21(9):1260-1271...cholecystokinin interneurons in the basal amygdala facilitates fear extinction. eNeuro. 6(6). PubMed (...to single-component cellular definitions (e.g. neurons defined by a single gene or projection). Intersectional...Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed excitation...excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PubMed (Link opens in...Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit...

  12. Cre-lox system

    Type
    Collection
    ...FRT sites) Mammalian Mombaerts 17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...newborn neurons Retroviral Luikart 66693 pRubiG-T2A-cre GFP and Cre expression in newborn neurons Retroviral...See also similar plasmids pSicoR PGK puro , pSicoR-Ef1a-mCh-Puro , pSicoR-Ef1a-mCh and pSicoR-mCh-empty...pIC-Cre Cre Bacterial Rajewsky 22776 MSCV CreERT2 puro Cre-ERT2 - Tamoxifen inducible Retroviral Jacks ...pLEW100cre-EP1 Cre Trypanosoma brucei Cross 34564 pMSCVpuro-Cre Cre 5'LTR Retroviral Ge 34565 pMSCVhygro-Cre...pmSyn1-EBFP-Cre Cre-EBFP fusion; Expression in neurons. synapsin AAV Zeng 51904 paavCAG-iCre iCre CAG ...

  13. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 NOT AVAILABLE YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter...promoter pCDH-EF1-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 ... promoter pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter...increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene of interest from the CB promoter...the mouse U6 promoter pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro 73022 Expresses Nluc and copGFP from the EF1 promoter...from the EF1 promoter pCDH-EF1s-Nluc-P2A-copGFP-T2A-Puro 73032 Expresses Nluc and copGFP from a truncated...

  14. Rett Syndrome

    Type
    Collection
    ..., an immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific...Background Rett Syndrome Rett syndrome is a neurodevelopmental disorder that presents in early toddlerhood...contrast to the sustained loss of skills in neurodegenerative conditions, the regressive period in Rett ...determines a minimally functional MECP2 that rescues neurological defects of Rett syndrome. (Link opens in a new... PMID: 24399845 Guy et al. 2007. Reversal of Neurological Defects in a Mouse Model of Rett Syndrome. Science...girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens in a new window) PMID...progressive encephalopathy in boys and MECP2 mutations. Neurology . 67, 164–166. (Link opens in a new window) PMID...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PDECGF, TP, hPD-ECGF UCN urocortin MGC129974, MGC129975, UI, UROC UCN2 urocortin 2 SRP, UCN-II, UCNI, UR...plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase receptor CD87, UPAR... V2R AZU1 azurocidin 1 AZAMP, AZU, CAP37, HBP, HUMAZUR, NAZC BDNF brain-derived neurotrophic factor MGC34632... CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor MGC1774 ...disease (pseudoglioma) EVR2, FEVR, ND NENF neuron derived neurotrophic factor CIR2, NEUDESIN, SCIRP10, SPUF ..., p75(NTR), p75NTR NMB neuromedin B MGC17211, MGC2277, MGC3936 NMBR neuromedin B receptor - NODAL nodal...NRP1 neuropilin 1 BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NP1, NRP, VEGF165R NRP2 neuropilin 2 MGC126574...

  19. Lentivirus Plasmids

    Type
    Collection
    ...Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid 24150...and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916...variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-...CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP...plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV...

  20. Lentiviral Prep Service

    Type
    Collection
    ...library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and...library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ...
Showing: 261 - 280 of 337 results