We narrowed to 1,056 results for: STI

...lead to under and overestimation of fusion protein activity. We encourage investigators to characterize ...Erik L. Snapp and Lindsey M. Costantini. "You underestimate the power of the Dark Side." --Darth Vader in...While Vader was referring to the evil side of a mystical "Force," this quote is equally applicable to many...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...most eukaryotic) proteins localize to chemically distinct subcellular environments, including the organelles...

...Woodgett. Public Library of Science (PLOS) created a stir earlier this year when it announced its data access...caused a storm as the organization left open the question of how much data was necessary and reasonable.... errors in assumptions, use of inappropriate statistical methods, omission of some data, etc.  The firestorm... lab has efficiently trawled their own data? A distinct (perhaps similarly impoverished lab) might apply...published, this data is usually condensed into a statistically evaluated set of values.  However, to evaluate...what was actually carried out. While no one is suggesting all data be stored in a retrievable format, we... images are said to be representative or the statistical variation is calculated and placed in the legend...

... focus on how to choose an advisor/principal investigator (PI) that will also serve as a good mentor. ...advisor is a good mentor? You have to ask a lot of questions. Personality and style conflicts are a common ... determining a good fit. Here are just a few suggestions for determining whether an advisor will be a ...potential advisor and don’t be shy about asking hard questions. Talk to as many lab members as possible. Try ...Ask alumni of the lab. Most labs have websites listing alumi and it is easy to use publication records...this blog post. This document includes a list of questions to ask your potential advisors and other related...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring" Don’t ignore warning signs If lab...

...overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story forward - and this ... that the superheroes win in the end. But we’re still invested in seeing how they do it.    Finding...your formal scientific communications, they are fantastic fodder for science communication. The trick is... the pieces that make you think, “Oh, that’s interesting! How did you solve that problem?” Those are the...big-picture problem, by asking your audience a simple question, like “Have you ever thought about how X works...conflict, you’re encouraging your audience to ask questions. Why didn’t the experiment work? What did those...preventing them from moving forward? Answering these questions will bring you and your audience - via the scientific...

...What tools are still missing from the optogenetics toolbox? Boyden: That's a good question. We've made a...about this latest optogenetics advance, the big questions that motivate his interest in neuroengineering...neuroengineering, and some of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering Addgene...compete against each other. Getting two-color stimulation has been a holy grail of the field. The problem...fundamental understanding of the brain, which has humanistic and philosophical implications. As an example... a lot of progress. I would say we still would like to find ways to make these tools more selective for...calcium or some other specific ion that would be interesting. It would also be great if you could make neural...

... so minimal experimental testing is required. So how can you do this? Testing all IFNs is completely unnecessary...are a matter of sensitivity. Therefore, the key question is not whether off-target effects exist, but what...simple: choose the least active SpCas9 variant that still efficiently cuts your on-target site. If a nuclease...). However, only the least active variant which still retains on-target activity (highlighted by a bold...higher-activity nucleases, the weakest variant that is still active can achieve off-target–free editing for this...Figure 2). Identify the least active variant that still shows the right amount of on-target editing. This.... (In the example in Figure 3, the least active still working variant was B-HypaR, thus variants with ...

...being able to ask follow up questions, or ask people to rephrase questions was key. I had also hoped that...that’s what keeps things interesting. And hopefully they’ll be just as interesting as the finished product...familiar with this, it’s a method for building and testing new products or methods, using metrics and feedback...webcams.  Build: I sent the participants interview questions they could answer, and I’d cut together a video...the office had partially reopened. But there were still many restrictions in place, most importantly, masks...would allow me to set up a camera in a room, but still have control over focus, zoom, stop/start record... to mount a battery to it if necessary. I could still control the camera from a distance if I needed to...

...Harvard Business Review article on procrastination recommends testing out how much time you can possibly...conceptual chunks: prioritization, planning, and procrastination. Time management through prioritiziation Every...option. Even if the full experiment fails, you’ll still have made small accomplishments each day that should...Eric before doing anything else. Preventing procrastination Even if you’ve done all the prioritizing and...and planning in the world, procrastination can easily prevent you from making progress on a task. For ...few things I’ve found useful to avoid such procrastination: Remove distractions: Probably the easiest...book, “Bored and Brilliant” for learning more fantastic reasons to remove yourself from distractions every...

...et al. "The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations....Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow...for improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila... Cre recombinase can be used alongside common, existing tools for fly research that use Flp recombination... to describe the self-renewal of cells in the intestinal crypt, and since then has become a powerful tool... researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow single...

...listeners to ask questions and record those that were asked. Reflect on the questions and come up with...Google.slides or Powerpoint file right now to capture interesting key results, striking images, creative ideas,... write itself when the time comes. 5. Choose interesting content Talk about something you know a lot about...commercialized, just talking about the possibility is interesting. Check out Joanne's Reddit AMA 6. Practice, practice...everybody loves a great animation. 8. Predict questions, prepare answers Speaking pros always have a few...always better than too long, and ensures time for questions. Don’t be tempted to cover too many things. One...this enough…more is almost never better. 10. Be enthusiastic You don’t have to emote beyond your comfort ...

... to the products of parental plasmid digestion (blue). Digestion with enzyme “B” detects primer multimerization.... Many thanks to our Guest Blogger Kristian Laursen! Kristian Laursen is a researcher at Cornell University...This post was contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis...can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach...perform alanine scanning mutagenesis or targeted substitution of key residues), and in SNP analysis (to introduce..., the template is removed by DpnI restriction digestion leaving behind only the mutagenized plasmid. Although... bases at the 3’-end interfere with plasmid reconstitution, and consequently Taq polymerases cannot be...

... µL size serotype testing) pAAV-CAG-GFP 50465 AAV8, 9 (20 µL size serotype testing) AAV-hSyn-EGFP ...size serotype testing) pAAV-CAG-GFP 50465 AAV1, 2, 5, rg (20 µL size serotype testing) pAAV-hSyn-EGFP...Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic...control AAV that scientists can use for serotype testing. These samples are available in various serotypes...encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid Serotype Name ...This variant exhibits high contrast between its resting state and its calcium-bound state, which was achieved...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

...Associate Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision... many tricks up their sleeves and most patients still don’t benefit from this approach. One central bottleneck...We've developed a new plasmid toolkit, the Broad Institute Target Accelerator Plasmid Collection, that we...have been two huge challenges preventing us from testing this hypothesis: There was no large scale, broadly...cellular pathways). It wasn’t clear whether gene-agnostic bioassays could accurately test for molecular ...variant function and cancer phenotypes. Broad Institute Target Accelerator Plasmid Collections To overcome...this first challenge, we’ve developed the Broad Institute Target Accelerator Plasmid Collections, now widely...

...David Root from the Broad Institute and Alex Chavez from the Wyss Institute were instrumental in helping...School, and Jonathan Ting’s lab from the Allen Institute have supported the AAV initiative from the beginning...at Addgene. Our Board helps keep our focus on assisting scientists and accelerating research and discovery...Team has been developing expertise in answering questions on virus and helping provide educational resources...word out about this new resource and providing fantastically useful educational content on viral vectors....internationally and be ready to provide extensive logistics support for export and shipping on dry ice. The...permission from relevant plasmid depositors and their institutions to distribute viral preps derived from plasmids...

...2):a001107. PMC PMID: 20182618 . Do you have suggestions for other plasmids that should be added to this...

...last reviewed: 10 September 2025 Do you have suggestions for other plasmids that should be added to this...

...lead to cystic fibrosis, one of the most prevalent inherited diseases. The authors investigated the role...One common strategy is protein overexpression — boosting levels so interactions aren't missed. But this...knock-ins maintain the native protein context, but they still depend on antibody-based detection or western blotting...binding to its complementary partner LgBiT, reconstitutes a fully functional luciferase enzyme (Figure...HiBiT provides a means to quickly answer research questions before creating knock-ins.  Figure 1: ...these variants of p53 shape chemosensitivity and mechanistic drug responses (Kamio et al., 2025). They designed... to characterize the proteasomal degradation of cystic fibrosis transmembrane conductance regulators (...

...on-target specificity. Design and testing of SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly ..., researchers from the Zhang lab at the Broad Institute, the Joung lab at Harvard Medical School, and ...activity of the Cas9 nuclease itself. Design and testing of eSpCas9 Looking at the structure of the Cas9... Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate Cas9 mutants...multiple genomic locations in HEK cells. Further testing of two of the mutants, SpCas9(K855A) and eSpCas9...interaction sbetween the non-target strand and Cas9, Kleinstiver et al disrupted interactions between Cas9 and...We hope one of you will be able to answer this question very soon! We look forward to seeing how the research...

...the first time, have lots of questions. To keep up with all these questions, Dr. Le Cong and Dr. Feng ...CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR", short-hand for ...developed a Google Forum to allow scientists to ask questions and get answers on the fly. But just like the ..., to organize the CRISPR forum's most popular questions and answers. Designing your CRISPR genome editing...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...the efficiency will be lower, although this might still be okay if you are introducing a selection marker...insert (gRNA) in pX330/pX335 with BbsI/AgeI double digestion. As a successful insertion will destroy the BbsI...

...list of Fragmid destination vectors currently available. Table 1: Fragmid destination vectors Organism...depicted. (Bottom) Schematic depicting available destination vectors for various delivery methods, along with... with compatible inserts. All destination vectors contain an ampicillin resistance cassette and an origin...components you want, simply assemble them into a destination vector via Golden Gate cloning, perform an exonuclease...sequence for every individual assembly. What destination vectors are available? What you’re building ...Organism Delivery Type Destination Vector Mammalian Lentivirus pRDA_512 (RSV-driven; lentiGuide-based...Mammalian Transposon pRDA_757 (Piggybac; constitutive expression) Mammalian Plasmid pRDA_791...