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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 658 results for: CHI;

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Showing: 421 - 450 of 658 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced...for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding sites within...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    ...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers... world. The research project was hard; I was researching the epidemiology of breast cancer and I loved...

  3. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}null{% endraw %}{% ...should not be undertaken lightly! Indeed, truly achieving the gold standard requires not one but two rounds...

  4. Finding Your Science Policy Path

    Type
    Blog Post
    ... target. There are a multitude of options for achieving success in a science policy career with many opportunities...organizations, working in student government, and teaching and mentoring others (see more in Figure 2). Starting...

  6. Typing CRISPR Systems

    Type
    Blog Post
    ... mammalian cells. Type IV Type IV is the middle child (like me!) of CRISPR types — weird and poorly understood...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...

  7. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...a slide, or whole organs. Because the tissue architecture is preserved, this technique allows you to not...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Cofsky et al., 2022; Osuka et al., 2018). Each matching DNA:RNA pair gives just enough stability for the...Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... inhibitor with a degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are ...plasmids from this paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for ...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...With both siRNA and shRNA, it can be difficult to achieve sufficient knockdown to enable robust assessment...S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation...

  16. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me...

  20. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...

  21. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick Putting on lab gloves ...

  22. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...community, we've partnered with distributors in China, Korea, Japan, India, and Brazil.  We've also created...

  29. Summer Fun at Addgene!

    Type
    Blog Post
    ...see even more of us? We’re very excited to be launching the official Addgene TikTok at the end of this...
Showing: 421 - 450 of 658 results