We narrowed to 954 results for: COMP

...positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific...

...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...organism. Studying cell mobility within these complex environments has never been easy and, for many ...labelling of cells using cre-lox is advantageous when compared to photoactivable or photoconvertible proteins...

...straightforward to learn and relatively cheap (compared to many other technologies and techniques in the...experiment work? What are the various tools and components necessary to run it? And, most importantly, what...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nature methods....

...fragments, allowing ordered assembly of multiple components into a destination vector. The first step in ...Oligonucleotides specifying the gRNA seed sequence should be compatible with type IIS enzyme BfuAI. Gateway cloning is...supply it with another plasmid.   Figure 3: Comparison of Multiplex Strategies including Standard PolIII-gRNA...Cas9-containing binary vectors. The system is compatible with both monocot and dicot plants. Liu Lab Golden...

...from most sequencing services, along with a more complete list of common sequencing primers. These lists...

...easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the...off-target editing and similar on-target editing when compared to the most commonly used Cas9 variant, SpCas9...Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore...

...are of interest to you, our readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our ...learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular, generating ...Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter...

...difficult to compare the performance of different nucleases. Nicola Patron’s lab compared the efficiency...more predictable and precise deletion products compared to monomeric Cas9’s. Read the paper in Nature...

...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this collection...upon, a wealth of history to learn from, and a compelling future to plan in the changing landscape of science...

...tags with defined 3D structures less likely to compromise protein folding and function. Using bioinformatic...GFP did not reduce yeast cell size or viability compared to untagged GFP overexpression, while the rest...cell size and growth rate, indicating that they compromised protein-protein interactions. If you’re looking...

...research and training is getting tighter and competition is fierce. However, I believe there are plenty...Washington University founded a successful consulting company called The Balsa Group. University of Pennsylvania... Consulting. I know of many schools that have completely student/postdoc-driven biotechnology clubs such...

...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...GreenGate can do is “save you half a year,” in comparison to more tedious and time-consuming traditional...traditional cloning methods, Lohmann said. The key component of Golden Gate and GreenGate are type IIS restriction...

... For more details, check out this comprehensive review comparing optogenetic CRISPR techniques (Matony...repeatability. The system developed by the Sata lab is composed of two fusion proteins: 1) the genomic anchor ...of transferrin receptor CD71 in HeLa cells.   Comparison of photoactivatable CRISPR strategies Publication...

...labels are obtained by independent audit, and companies need to pay to obtain them. There’s a database...you don’t see one, be an ACTivist and encourage companies to obtain these labels! Reusing plastic Historically...benchtop, high throughput washing devices that are compatible with a variety of pipette tips. This is part ...

...concentrations, incubation times, and recommended compatible reagents. Secondary antibodies must match the...

...bird feces, which get eaten by another snail, completing its lifecycle. Halloween genes The Halloween ...grasshoppers expressed different proteins in their brains compared to uninfected grasshoppers, including those linked...Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous Halloween...

...could take all of the credit, the success of the company is attributed to the entire Addgene family; the... on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as plenty of delicious...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular...CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout et al.,...

... come in the new year. I am proud of what we accomplished in 2023 and thrilled to be sharing our plans...addition, we released the MORF collection, a comprehensive transcription factor library, in lentiviral ... efficiencies typically adopted by for-profit companies, while making use of our nonprofit status to achieve...

...through biomedical research. The Camelid family is composed of camels, llamas, and alpacas (and their undomesticated...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...blog over the years and have described how they compare to other antibodies and affinity reagents. But ...

...change in pathology. If the disease pathology is complex, a model may be most effective. Once drug candidates...and generate physiologically relevant models. In complex diseases, it can aid in generation of multiple ...specificity of that target and validate the hit compound. The standard is to test the drug on models which...

...of this effort, members of the Rawlins Lab have compiled a new toolkit for performing genetics studies ...workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS...in high throughput screening for agrochemical compounds that have the potential to regulate the parasitization...

... expert (SME) at eScience Labs, an educational company that creates hands-on science lab kits and digital...with faculty members to make sure the courses encompass what they want to teach.” Besides working with...lab: write!” Caitlin did this by editing 15+ comprehensive exam proposals, a few PhD theses, and occasionally...

...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better...

...transfer in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required...efficiencies of stable gene transfer using SB100x were comparable to stable transduction efficiencies of integrating...

...CRISPR delivery systems, modifications to the RNA components, and challenging target genomes, such as the ...foremost, from the custom software used to design complex genetic circuitry to the downstream output. Tuesday...digital and analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also...

...labs today are semi-synthetic or fully synthetic compounds. Antibiotics can be categorized based on whether...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...make sure your plasmid and E. coli strain are compatible! Check out this list of common E. coli genotypes...

...used live virus (Cele et al., 2021). “We weren't completely shocked [with these results] because we were ... variant, which has only 9 different mutations compared to the wild-type, escaped neutralization. “That...SARS-CoV-2 variants, this study was the first to compare so many different strains. It was also the first...

...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...

...faithfully transcribes RNA to DNA with low error rates compared to other RTs. MarathonRT represents a major step...long-read cDNA sequencing and RNA sequencing of complex heterogeneous RNA samples. Furthermore, such high... restricted spatial tagging), to confirm the composition of proteins found at the telomere, and hope it...

...of their luciferase assay, they concluded that components in the buffer used for protein purification must...percentages (78-79% after two rounds of transduction.) Compared to CCP transduction, iTOP is at least four times...Astolfo et al. were eager to see if iTOP would be compatible with CRISPR/Cas9 genome editing. Although CRISPR...

... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then... Vice President. Her current responsibilities encompass overall leadership of the Cell Press editorial...

...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright...snaking through our cells’ nuclei--our chromatin is comprised of thousands of loops. These loops not only provide...

...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters (Zhang et al., 2019). How does...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...

...writing and this recent article published in PLOS Computational Biology (Frassl et al., 2018): “Science is increasingly...collaboratively writing a multi-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi...Simple Rules for a Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi...

... I asked participants to read short abstracts comparing the mortality rates between two controversial ...and biased. As a result, the control abstract comparing two fictional medical issues – chronic and acute...community. I brought along printed copies of studies comparing the filtration efficacy of different materials...

... of the world was locked down, a South African company wanted to test if a type of antibodies from an ... power of collaboration, which despite the competitiveness of cutting-edge research, is still one of the...need help with your scientific research. It’s completely free, of course. You can find more information...

.... Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized... genome. Many of these techniques require more complex DNA assemblies and improvements in these methodologies...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

...biosensor with two components - an enzyme that breaks down the HMO of interest into its component simple sugars...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...