We narrowed to 621 results for: Emb;

1. CRISPR 101: Making a Knock-In Cell Line

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   ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

2. Cpf1: A New Tool for CRISPR Genome Editing

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   ...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they...

3. Plasmids 101: Plasmid Incompatibility

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   ...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)...

4. Neuronal labeling with Spaghetti Monster

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   ... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above...

5. Transferable Skills Guide: External Collaborations

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   ...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective...

6. Deciphering the Mysteries of Behavior with Viral Vectors

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   ...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed...

7. Save Time with Transient Plant Leaf Transformations

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   ...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge...

8. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ...identified. While LY6A or other Ly6 gene family members are linked to viral susceptibility (mouse adenovirus...

9. New CRISPR Tools: Cas7-11 and PASTE

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   ...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development...

10. Antibodies 101: Immunoprecipitation

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    ...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases...

11. Viral Production at Addgene

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    ...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...

12. Hot Plasmids Spring 2024

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    ...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control...

13. AAV Titers: Where Do They Come From And What Do They Mean?

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    ...Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category...

14. Transferable Skills Guide: Career Planning Resources

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    ...clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc...

15. Career Insights: Technical Support Specialist

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    ...the larger biotech companies), and as far as I remember, it was the first time that I was absolutely convinced...

16. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron...

17. Viral Vectors 101: Pseudotyping

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    ...find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table...

18. Healthcare Consulting: A Door to the Business of Life Sciences

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    ... on the expertise of the senior leadership and members of their consulting staff. Who would enjoy healthcare...

19. Getting the Most from Your Lentiviral Transduction

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    ...., Rubinstein, M. “LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis...

20. Plasmids 101: Control Plasmids

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    ...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central...

21. Tips for Using FRET in Your Experiments

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    ...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf...

22. Harnessing Bacterial Toxins for Allelic Exchange

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    ...excellent efficiency using a commercial isothermal assembly master mix. Introduction of the strong transcriptional...

23. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets...

24. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ...running your analysis. In this case, I and other lab members had worked with lymphocytes before, so I knew where...

25. Transferable Skills Guide: Time Management

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    ...physically cross things out (it just feels good) and I remember things better when I write them down. Some people...

26. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...deliver these components to your target cells. Remember, efficient delivery of CRISPR components is essential...

27. Evolution of Lab Techniques

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    ...smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization of a radioactively...

28. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ...Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi:10.1038/nature.2015.18947...

29. Viral Vectors 101: AAV Variables That Matter

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    ...Attribution License. Within the nervous system, remember that some serotypes can be transported retrogradely...

30. Antibodies 101: Multiplex Immunofluorescence

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    ...this might be frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed...

31. Plasmids 101: Dimers and Multimers

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    ...control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which cannot distinguish...

32. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts used...

33. Building Global Connections with the International Mentorship Program USA-EUROPE

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    ...Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical School/Brigham and Women’s Hospital...

34. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning...

35. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...handling cell lines. Avoid discussion with other lab members, sneezing or coughing in the lab. The human mouth...

36. Finding Your Science Policy Path

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    ...backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical year or transition...

37. Quick Guide to All Things Lentivirus

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    ...liposome) or electrical (electric current forming membrane pores) transfection. These techniques are still...

38. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ...perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies...

39. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    ... cell mobility are essential processes during embryonic development. These are required for a growing ...

40. Custom CRISPR Screens & the Green Listed Software

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    ...screen. Currently, eleven published libraries are embedded. These were kindly donated by six different research...